Human Toll Like Receptor 5 (TLR-5) ELISA Kit | TLR-5 elisa kit

Human Toll Like Receptor 5 (TLR-5) ELISA Kit

Cat. Num. AAA269871

• Gene Names: TLR5; SLE1; TIL3; SLEB1; MELIOS
• Reactivity: Human
• Synonyms: Toll Like Receptor 5 (TLR-5); Human Toll Like Receptor 5 (TLR-5) ELISA Kit; TLR-5 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 858

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: Up to 0.06 ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TLR-5 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human TLR-5 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 215274239
• NCBI GeneID: 7100
• Molecular Weight: 97,834 Da
• NCBI Official Full Name: Toll-like receptor 5
• NCBI Official Synonym Full Names: toll-like receptor 5
• NCBI Official Symbol: TLR5
• NCBI Official Synonym Symbols: SLE1; TIL3; SLEB1; MELIOS
• NCBI Protein Information: toll-like receptor 5; toll/interleukin-1 receptor-like protein 3; systemic lupus erythematosus susceptibility 1
• UniProt Protein Name: Toll-like receptor 5
• UniProt Gene Name: TLR5
• UniProt Synonym Gene Names: TIL3
• UniProt Entry Name: TLR5_HUMAN

NCBI Description

This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009]

Uniprot Description

TLR5: Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1). Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1q41-q42

Cellular Component: plasma membrane; integral to membrane

Molecular Function: interleukin-1 receptor binding; transmembrane receptor activity

Biological Process: positive regulation of interleukin-8 production; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of cytokine secretion; positive regulation of nitric oxide biosynthetic process; male gonad development; defense response to bacterium; toll-like receptor signaling pathway; innate immune response; inflammatory response; positive regulation of toll-like receptor signaling pathway; toll-like receptor 10 signaling pathway

Disease: Legionnaire Disease, Susceptibility To; Systemic Lupus Erythematosus, Susceptibility To, 1; Melioidosis, Susceptibility To