Human TLR1 ELISA Kit | TLR1 elisa kit

Human TLR1 PicoKine ELISA Kit

Cat. Num. AAA177377

• Gene Names: TLR1; TIL; CD281; rsc786; TIL. LPRS5
• Reactivity: Human
• Synonyms: TLR1; Human TLR1 PicoKine ELISA Kit; Toll-like receptor 1; toll-like receptor 1; CD281; CD281 antigen; KIAA0012; LPRS5; rsc786; Similar to Drosophila Toll protein; TIL; TIL. LPRS5; Tlr1; TLR1_HUMAN; Toll (Drosophila) homolog; Toll like receptor 1; Toll like receptor 1 Precursor; Toll/Interleukin 1 receptor like; Toll/interleukin-1 receptor-like protein; TLR1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human TLR1
• Sequence Length: 786

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human TLR1 PicoKine ELISA Kit standard curve )

Related Product Information for TLR1 elisa kit

Principle of the assay: human TLR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TLR1 has been precoated onto 96-well plates. Standards(NSO, S22-N578) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TLR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TLR1 amount of sample captured in plate.
Background: TLR1 is a member of the Toll-like receptor family (TLR) of pattern recognition receptors of the innate immune system. TIR1 gene was mapped to 4p14 by fluorescence in situ hybridization. TLR1 recognizes pathogen-associated molecular pattern with a specificity for gram-positive bacteria. TLR1 has also been designated as CD281 (cluster of differentiation 281). TLR1 interacts with TLR2 to recognize the lipid configuration of the native mycobacterial lipoprotein as well as several triacylated lipopeptides.

NCBI and Uniprot Product Information

• NCBI GI #: 146291086
• NCBI GeneID: 7096
• UniProt Accession #: Q15399; O15452; Q32MK3; Q32MK4; Q9UG90; D1CS39; D1CS41
• Molecular Weight: 90,291 Da
• NCBI Official Full Name: Toll-like receptor 1
• NCBI Official Synonym Full Names: toll-like receptor 1
• NCBI Official Symbol: TLR1
• NCBI Official Synonym Symbols: TIL; CD281; rsc786; TIL. LPRS5
• NCBI Protein Information: toll-like receptor 1; toll/interleukin-1 receptor-like protein
• UniProt Protein Name: Toll-like receptor 1
• Protein Family: Toll-like receptor
• UniProt Gene Name: TLR1
• UniProt Synonym Gene Names: KIAA0012; TIL
• UniProt Entry Name: TLR1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TLR1: Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Binds MYD88 (via TIR domain). Interacts with CNPY3. Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2. Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Belongs to the Toll-like receptor family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: phagocytic vesicle membrane; membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; protein heterodimerization activity; receptor activity

Biological Process: MyD88-dependent toll-like receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; macrophage activation; regulation of cytokine secretion; positive regulation of interleukin-6 biosynthetic process; innate immune response; immune response; detection of triacylated bacterial lipoprotein; inflammatory response; signal transduction; toll-like receptor 1 signaling pathway

Disease: Leprosy, Susceptibility To, 5