Human Thyroxine Binding Globulin (TBG) ELISA Kit | TBG elisa kit

Human Thyroxine Binding Globulin (TBG) ELISA Kit

Cat. Num. AAA2022541

• Gene Names: SERPINA7; TBG
• Reactivity: Human
• Synonyms: Thyroxine Binding Globulin (TBG); Human Thyroxine Binding Globulin (TBG) ELISA Kit; SERPINA7; Serpin Peptidase Inhibitor Clade A Member 7; T4-binding globulin; TBG elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of TBG. No significant cross-reactivity or interference between TBG and analogues was observed.
• Sequence Length: 415

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.78 -50ng/mL
• Sensitivity: < 0.31ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TBG were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TBG were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TBG elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TBG in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to TBG. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to TBG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TBG elisa kit

Endocrinology

NCBI and Uniprot Product Information

• NCBI GI #: 205277441
• NCBI GeneID: 6906
• NCBI Accession #: NP_000345.2
• NCBI GenBank Nucleotide #: NM_000354.5
• UniProt Accession #: P05543; D3DUX1
• Molecular Weight: 46,325 Da
• NCBI Official Full Name: thyroxine-binding globulin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• NCBI Official Symbol: SERPINA7
• NCBI Official Synonym Symbols: TBG
• NCBI Protein Information: thyroxine-binding globulin
• UniProt Protein Name: Thyroxine-binding globulin
• UniProt Gene Name: SERPINA7
• UniProt Synonym Gene Names: TBG
• UniProt Entry Name: THBG_HUMAN

NCBI Description

There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.[provided by RefSeq, Jun 2012]

Uniprot Description

SERPINA7: Major thyroid hormone transport protein in serum. Defects in SERPINA7 are a cause of thyroxine-binding globulin deficiency (TBG deficiency). Mutations in the SERPINA7 gene can result as a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. Patients show, respectively, reduced or elevated protein- bound iodine but are euthyroid. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; hormone binding

Biological Process: response to drug; response to peptide hormone stimulus; aging; response to corticosterone stimulus; response to vitamin A; post-embryonic development