Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tankyrase-2 (TNKS2) Standard Curve.)
Human Tankyrase-2 (TNKS2) ELISA Kit | TNKS2 elisa kit
Human Tankyrase-2 (TNKS2) ELISA Kit
Gene Names
TNKS2; TNKL; ARTD6; TANK2; pART6; PARP5B; PARP5C; PARP-5b; PARP-5c
Reactivity
Human
Synonyms
Tankyrase-2 (TNKS2); Human Tankyrase-2 (TNKS2) ELISA Kit; TNKS2; PARP-5b; PARP-5c; PARP5B; PARP5C; TANK2; TNKL; tankyrase 2; TNKS2 elisa kit
Reactivity
Human
Specificity
Human Tankyrase-2 (TNKS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNKS2. No significant cross-reactivity or interference between Human TNKS2 and analogues was observed.
Sequence Length
1166
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tankyrase-2 (TNKS2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Tankyrase-2 (TNKS2) Standard Curve.)
Related Product Information for TNKS2 elisa kit
Description: This Human Tankyrase-2 (TNKS2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNKS2 in samples. An antibody specific for TNKS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNKS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNKS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNKS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The poly(ADP-ribose) polymerase (PARP) tankyrase-1 contains an ankyrin-repeat domain that binds to various partners, including the telomeric protein TRF1 (telomere-repeat-binding factor 1) and the vesicular protein IRAP (insulin-responsive aminopeptidase). TRF1 binding recruits tankyrase-1 to telomeres and allows its PARP activity to regulate telomere homoeostasis. By contrast, IRAP binding and the Golgi co-localization of tankyrase-1 with IRAP might allow tankyrase-1 to affect the targeting ofIRAP-containing vesicles. A closely related protein, tankyrase-2, has also been implicated in vesicular targeting. Unlike tankyrase-1, tankyrase-2 has not been shown to have PARP activity. In addition, it has not been implicated in telomere homoeostasis, because it did not interact with TRF1 in previous studies.
Background: The poly(ADP-ribose) polymerase (PARP) tankyrase-1 contains an ankyrin-repeat domain that binds to various partners, including the telomeric protein TRF1 (telomere-repeat-binding factor 1) and the vesicular protein IRAP (insulin-responsive aminopeptidase). TRF1 binding recruits tankyrase-1 to telomeres and allows its PARP activity to regulate telomere homoeostasis. By contrast, IRAP binding and the Golgi co-localization of tankyrase-1 with IRAP might allow tankyrase-1 to affect the targeting ofIRAP-containing vesicles. A closely related protein, tankyrase-2, has also been implicated in vesicular targeting. Unlike tankyrase-1, tankyrase-2 has not been shown to have PARP activity. In addition, it has not been implicated in telomere homoeostasis, because it did not interact with TRF1 in previous studies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
tankyrase 2
NCBI Official Symbol
TNKS2
NCBI Official Synonym Symbols
TNKL; ARTD6; TANK2; pART6; PARP5B; PARP5C; PARP-5b; PARP-5c
NCBI Protein Information
poly [ADP-ribose] polymerase tankyrase-2; tankyrase-2
UniProt Protein Name
Tankyrase-2
Protein Family
UniProt Gene Name
TNKS2
UniProt Synonym Gene Names
PARP5B; TANK2; TNKL; TANK2; ARTD6
UniProt Entry Name
TNKS2_HUMAN
Research Articles on TNKS2
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Product Notes
The Human TNKS2 tnks2 (Catalog #AAA9713861) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713861 ELISA Kit recognizes Human TNKS2. It is sometimes possible for the material contained within the vial of "Tankyrase-2 (TNKS2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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