Human Tachykinin, Precursor 1 (TAC1) ELISA Kit | TAC1 elisa kit

Human Tachykinin, Precursor 1 (TAC1) ELISA Kit

Cat. Num. AAA2700584

• Gene Names: TAC1; NK2; NPK; NKNA; TAC2; Hs.2563
• Reactivity: Human
• Synonyms: Tachykinin; Precursor 1 (TAC1); Human Tachykinin; Precursor 1 (TAC1) ELISA Kit; NK2; NKNA; NKA; TAC2; •PPT; Protachykinin-1; Substance P; Neurokinin A; Neuromedin L; Substance K; Neuropeptide K; Neuropeptide gamma; C-terminal-flanking peptide; TAC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Tachykinin, Precursor 1 (TAC1).; No significant cross-reactivity or interference between Tachykinin, Precursor 1 (TAC1) and analogues was observed.
• Sequence Length: 129

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 123.46-10000pg/mL
• Sensitivity: < 52.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tachykinin, Precursor 1 (TAC1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAC1 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Tachykinin, Precursor 1 (TAC1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Tachykinin, Precursor 1 (TAC1) and unlabeled Tachykinin, Precursor 1 (TAC1) (Standards or samples) with the pre-coated antibody specific to Tachykinin, Precursor 1 (TAC1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Tachykinin, Precursor 1 (TAC1) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 17390092
• NCBI GeneID: 6863
• Molecular Weight: 11,376 Da
• NCBI Official Full Name: Tachykinin, 1
• NCBI Official Synonym Full Names: tachykinin precursor 1
• NCBI Official Symbol: TAC1
• NCBI Official Synonym Symbols: NK2; NPK; NKNA; TAC2; Hs.2563
• NCBI Protein Information: protachykinin-1
• UniProt Protein Name: Protachykinin-1
• Protein Family: Transcriptional regulator
• UniProt Gene Name: TAC1
• UniProt Synonym Gene Names: NKA; NKNA; TAC2; NKA; NPK

NCBI Description

This gene encodes four products of the tachykinin peptide hormone family, substance P and neurokinin A, as well as the related peptides, neuropeptide K and neuropeptide gamma. These hormones are thought to function as neurotransmitters which interact with nerve receptors and smooth muscle cells. They are known to induce behavioral responses and function as vasodilators and secretagogues. Substance P is an antimicrobial peptide with antibacterial and antifungal properties. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

Uniprot Description

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles.