Human SYT3 (Synaptotagmin-3) ELISA Kit | SYT3 elisa kit

Human SYT3 (Synaptotagmin-3) ELISA Kit

Cat. Num. AAA7612196

• Gene Names: SYT3; SytIII
• Reactivity: Human
• Synonyms: SYT3 (Synaptotagmin-3); Human SYT3 (Synaptotagmin-3) ELISA Kit; SYT3; Synaptotagmin-3; Synaptotagmin III; SytIII; SYT3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SYT3. No significant cross-reactivity or interference between SYT3 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SYT3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 119592297
• NCBI GeneID: 84258
• UniProt Accession #: Q9BQG1; Q8N5Z1; Q8N640
• Molecular Weight: 63,304 Da
• NCBI Official Full Name: synaptotagmin III, isoform CRA_a
• NCBI Official Synonym Full Names: synaptotagmin 3
• NCBI Official Symbol: SYT3
• NCBI Official Synonym Symbols: SytIII
• NCBI Protein Information: synaptotagmin-3
• UniProt Protein Name: Synaptotagmin-3
• Protein Family: Synaptotagmin
• UniProt Gene Name: SYT3
• UniProt Synonym Gene Names: SytIII

Uniprot Description

Ca2+ sensor involved in Ca2+-dependent exocytosis of secretory vesicles through Ca2+ and phospholipid binding to the C2 domain. Ca2+ induces binding of the C2-domains to phospholipid membranes and to assembled SNARE-complexes; both actions contribute to triggering exocytosis (). Plays a role in dendrite formation by melanocytes (PubMed:23999003).