Human Syntaxin 2 (STX2) ELISA Kit | STX2 elisa kit

Human Syntaxin 2 (STX2) ELISA Kit

Cat. Num. AAA453029

• Gene Names: STX2; EPM; EPIM; STX2A; STX2B; STX2C
• Reactivity: Human
• Synonyms: Syntaxin 2 (STX2); Human Syntaxin 2 (STX2) ELISA Kit; EPIM; EPM; STX2A; STX2B; STX2C; Epimorphin; STX2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of STX2. No significant cross-reactivity or interference between STX2 and analogues was observed.
• Sequence Length: 287

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.109ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level STX2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level STX2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STX2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of STX2 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to STX2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to STX2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STX2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of STX2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37577287
• NCBI GeneID: 2054
• NCBI Accession #: NP_001971.2
• NCBI GenBank Nucleotide #: NM_001980.3
• UniProt Accession #: P32856; Q86VW8
• Molecular Weight: 32,425 Da
• NCBI Official Full Name: syntaxin-2 isoform 1
• NCBI Official Synonym Full Names: syntaxin 2
• NCBI Official Symbol: STX2
• NCBI Official Synonym Symbols: EPM; EPIM; STX2A; STX2B; STX2C
• NCBI Protein Information: syntaxin-2
• UniProt Protein Name: Syntaxin-2
• Protein Family: Syntaxin
• UniProt Gene Name: STX2
• UniProt Synonym Gene Names: EPIM; STX2A; STX2B; STX2C

NCBI Description

The product of this gene belongs to the syntaxin/epimorphin family of proteins. The syntaxins are a large protein family implicated in the targeting and fusion of intracellular transport vesicles. The product of this gene regulates epithelial-mesenchymal interactions and epithelial cell morphogenesis and activation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Essential for epithelial morphogenesis. May mediate Ca2+-regulation of exocytosis acrosomal reaction in sperm.