Human SYNGAP1 (Synaptic Ras GTPase-activating protein 1) ELISA Kit | SYNGAP1 elisa kit

Human SYNGAP1 (Synaptic Ras GTPase-activating protein 1) ELISA Kit

Cat. Num. AAA7608141

• Gene Names: SYNGAP1; MRD5; RASA1; RASA5; SYNGAP
• Reactivity: Human
• Synonyms: SYNGAP1 (Synaptic Ras GTPase-activating protein 1); Human SYNGAP1 (Synaptic Ras GTPase-activating protein 1) ELISA Kit; KIAA1938; MRD5; Neuronal RasGAP; RASA1; RASA5; Synaptic Ras GAP 1; SYNGAP; SYNGAP1; SYNGAP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SYNGAP1. No significant cross-reactivity or interference between SYNGAP1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SYNGAP1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 150421676
• NCBI GeneID: 8831
• UniProt Accession #: Q96PV0; Q8TCS2; Q9UGE2; A2AB17; A2BEL6; A2BEL7; A8MQC4
• Molecular Weight: 1343
• NCBI Official Full Name: Ras/Rap GTPase-activating protein SynGAP
• NCBI Official Synonym Full Names: synaptic Ras GTPase activating protein 1
• NCBI Official Symbol: SYNGAP1
• NCBI Official Synonym Symbols: MRD5; RASA1; RASA5; SYNGAP
• NCBI Protein Information: ras/Rap GTPase-activating protein SynGAP; neuronal RasGAP; Ras GTPase-activating protein SynGAP; synaptic Ras GTPase-activating protein 1; synaptic Ras GTPase activating protein, 135kDa; synaptic Ras GTPase activating protein 1 homolog
• UniProt Protein Name: Ras/Rap GTPase-activating protein SynGAP
• Protein Family: Ras/Rap GTPase-activating protein
• UniProt Gene Name: SYNGAP1
• UniProt Synonym Gene Names: KIAA1938; Synaptic Ras-GAP 1
• UniProt Entry Name: SYGP1_HUMAN

NCBI Description

The protein encoded by this gene is a major component of the postsynaptic density (PSD), a group of proteins found associated with NMDA receptors at synapses. The encoded protein is phosphorylated by calmodulin-dependent protein kinase II and dephosphorylated by NMDA receptor activation. Defects in this gene are a cause of mental retardation autosomal dominant type 5 (MRD5). [provided by RefSeq, Dec 2009]

Uniprot Description

SynGAP: an excitatory synapse-specific RasGAP. Member of the NMDAR signaling complex in excitatory synapses it may play a role in NMDAR-dependent control of AMPAR potentiation and synaptic plasticity. ERK activation is up-regulated in neurons from SynGAP knockout mice, whereas P38 MAPK function is depressed. May play a critical role in the regulation of neuronal MAPK signaling, AMPAR membrane trafficking, and excitatory synaptic transmission. Interacts with CAMK2A/CAMK2B and MPDZ. Phosphorylated by CaM-kinase II. Dephosphorylated upon NMDA receptor activation or SYNGAP1/MPDZ complex disruption. Contains a PH domain and a Ras-GAP domain. Two alternatively spliced isoforms have been described.

Protein type: GAPs, Ras; GAPs

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; dendritic shaft

Molecular Function: SH3 domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; regulation of MAPKKK cascade; regulation of synapse structure and activity; Ras protein signal transduction; dendrite development; regulation of synaptic plasticity; visual learning; negative regulation of Ras protein signal transduction; negative regulation of axonogenesis; negative regulation of neuron apoptosis; pattern specification process; receptor clustering

Disease: Mental Retardation, Autosomal Dominant 5