Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Statherin (STATH) Standard Curve.)
Human Statherin (STATH) ELISA Kit | STATH elisa kit
Human Statherin (STATH) ELISA Kit
Gene Names
STATH; STR
Reactivity
Human
Synonyms
Statherin (STATH); Human Statherin (STATH) ELISA Kit; STATH; STR; STATH elisa kit
Reactivity
Human
Specificity
Human Statherin (STATH) ELISA Kit has high sensitivity and excellent specificity for detection of Human STATH. No significant cross-reactivity or interference between Human STATH and analogues was observed.
Sequence Length
62
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Statherin (STATH) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Statherin (STATH) Standard Curve.)
Related Product Information for STATH elisa kit
Description: This Human Statherin (STATH) ELISA Kit employs a two-site sandwich ELISA to quantitate STATH in samples. An antibody specific for STATH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any STATH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for STATH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of STATH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Statherin may play an important role in the maintenance of oral health since, like the proline-rich proteins, it binds calcium and inhibits crystal growth. Statherin also inhibits spontaneous precipitation of calcium phosphate salts. Thus, statherin and PRPs may prevent build-up of harmful deposits in the salivary glands and on the tooth surfaces. Statherin is a highly stable salivary protein of low molecular mass (5,380). Sabatini et al. synthesized mixed oligonucleotides based on the known amino acid sequence of statherin and used these to screen a cDNA library constructed from human parotid gland mRNA. A study of DNA from human-hamster somatic cell hybrids indicated that statherin is coded by a single-copy gene located in region 4q11-q13. Dentinogenesis imperfectaand perhaps juvenile periodontitis are coded in the same region.
Background: Statherin may play an important role in the maintenance of oral health since, like the proline-rich proteins, it binds calcium and inhibits crystal growth. Statherin also inhibits spontaneous precipitation of calcium phosphate salts. Thus, statherin and PRPs may prevent build-up of harmful deposits in the salivary glands and on the tooth surfaces. Statherin is a highly stable salivary protein of low molecular mass (5,380). Sabatini et al. synthesized mixed oligonucleotides based on the known amino acid sequence of statherin and used these to screen a cDNA library constructed from human parotid gland mRNA. A study of DNA from human-hamster somatic cell hybrids indicated that statherin is coded by a single-copy gene located in region 4q11-q13. Dentinogenesis imperfectaand perhaps juvenile periodontitis are coded in the same region.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
statherin isoform a
NCBI Official Synonym Full Names
statherin
NCBI Official Symbol
STATH
NCBI Official Synonym Symbols
STR
NCBI Protein Information
statherin
UniProt Protein Name
Statherin
Protein Family
UniProt Gene Name
STATH
UniProt Entry Name
STAT_HUMAN
Uniprot Description
STATH: Salivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface. Belongs to the histatin/statherin family.
Protein type: Secreted; Secreted, signal peptide; Extracellular matrix
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: extracellular region
Molecular Function: protein binding; hydroxyapatite binding; structural constituent of tooth enamel; extracellular matrix constituent, lubricant activity
Biological Process: negative regulation of bone mineralization; ossification; biomineral formation; saliva secretion
Research Articles on STATH
Similar Products
Product Notes
The Human STATH stath (Catalog #AAA9714048) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714048 ELISA Kit recognizes Human STATH. It is sometimes possible for the material contained within the vial of "Statherin (STATH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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