Human Splicing factor 3A subunit 3, SF3A3 ELISA Kit | SF3A3 elisa kit

Human Splicing factor 3A subunit 3, SF3A3 ELISA Kit

Cat. Num. AAA2602705

• Gene Names: SF3A3; PRP9; PRPF9; SAP61; SF3a60
• Reactivity: Human
• Synonyms: Splicing factor 3A subunit 3; SF3A3; Human Splicing factor 3A subunit 3; SF3A3 ELISA Kit; SF3A3 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant SF3A3 concentration
• Assay Type: Sandwich
• Detection Range: 20 ng/ml-0.312 ng/ml
• Sensitivity: 0.06 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SF3A3 elisa kit

Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SF3A3 monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 5803167
• NCBI GeneID: 10946
• NCBI Accession #: NP_006793.1
• NCBI GenBank Nucleotide #: NM_006802.2
• UniProt Accession #: Q12874; Q15460; Q5VT87; D3DPT5
• Molecular Weight: 58,849 Da
• NCBI Official Full Name: splicing factor 3A subunit 3
• NCBI Official Synonym Full Names: splicing factor 3a, subunit 3, 60kDa
• NCBI Official Symbol: SF3A3
• NCBI Official Synonym Symbols: PRP9; PRPF9; SAP61; SF3a60
• NCBI Protein Information: splicing factor 3A subunit 3; SAP 61; spliceosome associated protein 61; spliceosome-associated protein 61; splicing factor 3a, subunit 3, 60kD; pre-mRNA splicing factor SF3a (60kD)
• UniProt Protein Name: Splicing factor 3A subunit 3
• UniProt Gene Name: SF3A3
• UniProt Synonym Gene Names: SAP61; SAP 61
• UniProt Entry Name: SF3A3_HUMAN

NCBI Description

This gene encodes subunit 3 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. Subunit 3 interacts with subunit 1 through its amino-terminus while the zinc finger domain of subunit 3 plays a role in its binding to the 15S U2 snRNP. This gene has a pseudogene on chromosome 20. [provided by RefSeq, Jul 2008]

Uniprot Description

SF3A3: subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.

Protein type: Spliceosome; RNA-binding; Motility/polarity/chemotaxis; RNA splicing

Chromosomal Location of Human Ortholog: 1p34.3

Cellular Component: nucleoplasm; spliceosome; nuclear speck

Molecular Function: protein binding; zinc ion binding

Biological Process: nuclear mRNA 3'-splice site recognition; RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; RNA splicing; gene expression; mRNA processing