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Human Sperm-associated antigen 17 (SPAG17) ELISA Kit | SPAG17 elisa kit

Human Sperm-associated antigen 17 (SPAG17) ELISA Kit

Gene Names
SPAG17; PF6; CT143
Reactivity
Human
Synonyms
Sperm-associated antigen 17 (SPAG17); Human Sperm-associated antigen 17 (SPAG17) ELISA Kit; DKFZp434B0610; FLJ34497; FLJ44343; FLJ44353; PF6; RP4-776P7.2; projection protein PF6; SPAG17 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SPAG17. No significant cross-reactivity or interference between Human SPAG17 and analogues was observed.
Sequence Length
2223
Assay Type
Quantitive Sandwich
Samples
Serum, Plasma, Other Biological Fluids
Detection Wavelength
450 nm
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%)
= SD/meanX100
Intra-Assay
CV
Inter-Assay
CV
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for SPAG17 elisa kit
This assay employs a two-site sandwich ELISA to quantitate SPAG17 in samples. An antibody specific for SPAG17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPAG17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPAG17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPAG17 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
251,742 Da
NCBI Official Full Name
sperm-associated antigen 17
NCBI Official Synonym Full Names
sperm associated antigen 17
NCBI Official Symbol
SPAG17
NCBI Official Synonym Symbols
PF6; CT143
NCBI Protein Information
sperm-associated antigen 17
UniProt Protein Name
Sperm-associated antigen 17
Protein Family
UniProt Gene Name
SPAG17

NCBI Description

This gene encodes a central pair protein present in the axonemes of cells with a "9 + 2" organization of microtubules. The encoded protein is required for the proper function of the axoneme. Mutations in the orthologous gene in mice lead to primary ciliary dyskinesia characterized by immotile nasal and tracheal cilia, reduced clearance of nasal mucus, profound respiratory distress, hydrocephalus, and neonatal lethality within twelve hours of birth due to impaired airway mucociliary clearance. Single-nucleotide polymorphisms in this gene are associated with human height and targeted mutations lead to skeletal malformations affecting the limbs in mice, suggesting a role for this gene in skeletal development. [provided by RefSeq, Feb 2017]

Uniprot Description

Component of the central pair apparatus of ciliary axonemes. Plays a critical role in the function and structure of motile cilia. May play a role in endochondral bone formation, most likely because of a function in primary cilia of chondrocytes and osteoblasts.

Research Articles on SPAG17

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Product Notes

The Human SPAG17 spag17 (Catalog #AAA287446) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287446 ELISA Kit recognizes Human SPAG17. It is sometimes possible for the material contained within the vial of "Sperm-associated antigen 17 (SPAG17), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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