Human Specificity Protein 1 (Sp1) ELISA Kit | Sp1 elisa kit

Human Specificity Protein 1 (Sp1)(Sandwich ELISA) ELISA Kit

Cat. Num. AAA2125318

• Reactivity: Human
• Applications: ELISA
• Synonyms: Specificity Protein 1 (Sp1); Human Specificity Protein 1 (Sp1)(Sandwich ELISA) ELISA Kit; High Sensitive ELISA Kit for Specificity Protein 1 (Sp1); Sp1/Specificity Protein 1; TSFP1; Sp1 Transcription Factor; Sp1 elisa kit

• Reactivity: Human
• Specificity: Reactive with Human Specificity Protein 1 (Sp1); This assay has high sensitivity and excellent specificity for detection of High Sensitive Specificity Protein 1 (Sp1). No significant cross-reactivity or interference between High Sensitive Specificity Protein 1 (Sp1) and analogues was observed.

• Applicable Applications for Sp1 elisa kit: ELISA (EIA)
• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Assay Type: Sandwich ELISA-High Sensitive
• Sample Types: tissue homogenates, cell lysates and other biological fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: 31pg/mL
• Detection Method: Double-antibody Sandwich
• Assay Length: 3 hours
• Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Specificity Protein 1 (Sp1) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Specificity Protein 1 (Sp1) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100.; Intra-Assay: CV<10%.; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to X-Ray Repair Cross Complementing 6 (XRCC6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the X-Ray Repair Cross Complementing 6 (XRCC6) level in the sample or standard.
• Assay Procedure Summary: 1. Prepare all reagents, samples and standards; 2. Add 100ul standard or sample to each well. Incubate 2 hours at 37 degree C; 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37 degree C; 4. Aspirate and wash 3 times; 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C; 6. Aspirate and wash 5 times; 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37 degree C; 8. Add 50ul Stop Solution. Read at 450nm immediately.
• Preparation and Storage: Store at 4 degree C for 1 month; -20 degree C for long-term (within shelf life); The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Product Categories/Family for Sp1 elisa kit

Signal transduction; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 352962149
• NCBI GeneID: 6667
• NCBI Accession #: NP_001238754.1
• NCBI GenBank Nucleotide #: NM_001251825.1
• UniProt Accession #: P08047; Q86TN8; Q9H3Q5; Q9NR51; Q9NY21; Q9NYE7
• Molecular Weight: 80,693 Da
• NCBI Official Full Name: transcription factor Sp1 isoform c
• NCBI Official Synonym Full Names: Sp1 transcription factor
• NCBI Official Symbol: SP1
• NCBI Protein Information: transcription factor Sp1; specificity protein 1
• UniProt Protein Name: Transcription factor Sp1
• Protein Family: Sex-specific storage-protein
• UniProt Gene Name: SP1
• UniProt Synonym Gene Names: TSFP1
• UniProt Entry Name: SP1_HUMAN

NCBI Description

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

SP1: a transcription factor of the Sp1 C2H2-type zinc-finger protein family. Phosphorylated and activated by MAPK. Dephosphorylation by PTEN inhibits DNA binding. Binds to p38 in the nucleus. Interacts with Huntingtin and TAFII130. Transcriptional activity of SP1 and TAFII130 disrupted in early Huntingtin's Disease.

Protein type: DNA-binding; Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 12q13.1

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: protein C-terminus binding; histone acetyltransferase binding; protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; metal ion binding; histone deacetylase binding; double-stranded DNA binding; bHLH transcription factor binding; transcription factor binding; transcription factor activity

Biological Process: transcription initiation from RNA polymerase II promoter; ossification; embryonic placenta development; transcription, DNA-dependent; viral reproduction; megakaryocyte differentiation; embryonic skeletal development; positive regulation of transcription, DNA-dependent; embryonic process involved in female pregnancy; rhythmic process; cellular lipid metabolic process; enucleate erythrocyte differentiation; liver development; trophectodermal cell differentiation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; lung development