Human SPARC ELISA Kit | SPARC elisa kit

Human SPARC PicoKine ELISA Kit

Cat. Num. AAA177339

• Gene Names: SPARC; ON
• Reactivity: Human
• Synonyms: SPARC; Human SPARC PicoKine ELISA Kit; secreted protein; acidic; cysteine-rich (osteonectin); AA517111; Basement membrane protein 40; Basement-membrane protein 40; BM 40; BM-40; BM40; Cysteine rich protein; MGC128090; ON; Osteonectin; Secreted acidic cystein rich glycoprotein; Secreted protein acidic and rich in cysteine; Secreted protein acidic cysteine rich (osteonectin); Secreted protein acidic cysteine rich; SPRC; SPRC_HUMAN; SPARC elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human SPARC
• Sequence Length: 303

• Samples: Cell culture supernates, serum and human milk
• Assay Type: Sandwich
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: < 20pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human SPARC PicoKine ELISA Kit standard curve )

Related Product Information for SPARC elisa kit

Principle of the assay: human SPARC ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SPARC has been precoated onto 96-well plates. Standards(NSO, A18-I303) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SPARC is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human SPARC amount of sample captured in plate.
Background: SPARC(secreted protein acidic and rich in cysteine), also known as Osteonectin, is a protein that in humans is encoded by the SPARC gene. The human SPARC gene is 26.5 kb long, and contains 10 exons and 9 introns and is located on chromosome 5q31-q33. SPARC is a glycoprotein of 40 kD. SPARC is an acidic, cysteine-rich glycoprotein consisting of a single polypeptide chain that can be broken into 4 domains: 1) an Ca++ binding domains near the glutamic acidic-rich region at the amino terminus (domain I), 2) a cysteine- rich (domain II), 3) a hydrophilic region (domain III) and 4) an EF hand motif at the carboxy terminus region (domain IV). Osteonectin is a glycoprotein in the bone that binds sodium. It is secreted by osteoblasts during bone formation, initiating mineralization and promoting mineral crystal formation. Osteonectin also shows affinity for collagen in addition to bone mineral calcium. A correlation between osteonectin over expression and ampullary cancers and chronic pancreatitis has been found.

NCBI and Uniprot Product Information

• NCBI GI #: 129283
• NCBI GeneID: 6678
• UniProt Accession #: P09486; Q6IBK4; D3DQH9
• Molecular Weight: 34,632 Da
• NCBI Official Full Name: SPARC
• NCBI Official Synonym Full Names: secreted protein, acidic, cysteine-rich (osteonectin)
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON
• NCBI Protein Information: SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• UniProt Protein Name: SPARC
• Protein Family: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; cell surface; nuclear matrix; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: platelet activation; receptor-mediated endocytosis; response to peptide hormone stimulus; response to gravity; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; response to cadmium ion; response to ethanol; platelet degranulation; response to cytokine stimulus; response to lead ion; negative regulation of endothelial cell proliferation; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii