Human Sodium/potassium transporting ATPase subunit Alpha 3 (ATP1A3) ELISA Kit | ATP1A3 elisa kit

Human Sodium/potassium transporting ATPase subunit Alpha 3 (ATP1A3) ELISA Kit

Cat. Num. AAA7220862

• Gene Names: ATP1A3; RDP; AHC2; DYT12
• Reactivity: Human
• Synonyms: Sodium/potassium transporting ATPase subunit Alpha 3 (ATP1A3); Human Sodium/potassium transporting ATPase subunit Alpha 3 (ATP1A3) ELISA Kit; ATP1A3 elisa kit

• Reactivity: Human

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP1A3 elisa kit

Intended Uses: This ATP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ATP. This ELISA kit for research use only!

Principle of the Assay: ATP ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATP antibody and an ATP-HRP conjugate. The assay sample and buffer are incubated together with ATP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP concentration since ATP from samples and ATP-HRP conjugate compete for the anti-ATP antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP from the sample, fewer sites are left to bind ATP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATP1A3 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 371940940
• NCBI GeneID: 478
• NCBI Accession #: NP_001243143.1
• NCBI GenBank Nucleotide #: NM_001256214.1
• Molecular Weight: 113,059 Da
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit alpha-3 isoform 3
• NCBI Official Synonym Full Names: ATPase, Na+/K+ transporting, alpha 3 polypeptide
• NCBI Official Symbol: ATP1A3
• NCBI Official Synonym Symbols: RDP; AHC2; DYT12
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit alpha-3; Na+/K+ ATPase 3; sodium pump subunit alpha-3; Na(+)/K(+) ATPase alpha-3 subunit; Na(+)/K(+) ATPase alpha(III) subunit; sodium-potassium-ATPase, alpha 3 polypeptide; sodium-potassium ATPase catalytic subunit alpha-3; sodium/potassium-transporting ATPase alpha-3 chain
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit alpha-3
• Protein Family: Sodium/potassium-transporting ATPase
• UniProt Gene Name: ATP1A3
• UniProt Synonym Gene Names: Na(+)/K(+) ATPase alpha-3 subunit
• UniProt Entry Name: AT1A3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

ATP1A3: This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. Defects in ATP1A3 are the cause of dystonia type 12 (DYT12); also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant dystonia- parkinsonism disorder. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. DYT12 patients develop dystonia and parkinsonism between 15 and 45 years of age. The disease is characterized by an unusually rapid evolution of signs and symptoms. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.

Protein type: Transporter, ion channel; EC 3.6.3.9; Endoplasmic reticulum; Hydrolase; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 19q13.31

Cellular Component: Golgi apparatus; axon; endoplasmic reticulum; plasma membrane; integral to membrane; synapse; nucleus; sodium:potassium-exchanging ATPase complex; myelin sheath; vesicle

Molecular Function: chaperone binding; metal ion binding; sodium:potassium-exchanging ATPase activity; D1 dopamine receptor binding; ATP binding

Biological Process: response to drug; cellular sodium ion homeostasis; potassium ion import; adult locomotory behavior; ionotropic glutamate receptor signaling pathway; metabolic process; visual learning; cellular potassium ion homeostasis; transmembrane transport; memory

Disease: Dystonia 12; Cerebellar Ataxia, Areflexia, Pes Cavus, Optic Atrophy, And Sensorineural Hearing Loss; Alternating Hemiplegia Of Childhood 2