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Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit | SLC10A1 elisa kit

Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit

Gene Names
SLC10A1; NTCP
Reactivity
Human
Synonyms
Sodium/bile acid cotransporter (SLC10A1); Human Sodium/bile acid cotransporter (SLC10A1) ELISA Kit; NTCP; Na/taurocholate cotransporting polypeptide|growth-inhibiting protein 29|sodium/bile acid cotransporter|sodium/taurocholate cotransporter|solute carrier family 10; member 1; SLC10A1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human SLC10A1. No significant cross-reactivity or interference between Human SLC10A1 and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate SLC10A1 in samples. An antibody specific for SLC10A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC10A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC10A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC10A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
38,119 Da
NCBI Official Full Name
Sodium/bile acid cotransporter
NCBI Official Synonym Full Names
solute carrier family 10 (sodium/bile acid cotransporter), member 1
NCBI Official Symbol
SLC10A1
NCBI Official Synonym Symbols
NTCP
NCBI Protein Information
sodium/bile acid cotransporter; growth-inhibiting protein 29; Na(+)/bile acid cotransporter; sodium/taurocholate cotransporter; solute carrier family 10 member 1; Na(+)/taurocholate transport protein; cell growth-inhibiting gene 29 protein; Na/taurocholat
UniProt Protein Name
Sodium/bile acid cotransporter
UniProt Gene Name
SLC10A1
UniProt Synonym Gene Names
NTCP
UniProt Entry Name
NTCP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the sodium/bile acid cotransporter family, which are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids; the ileal sodium/bile acid cotransporter with an apical cell localization that absorbs bile acids from the intestinal lumen, bile duct and kidney, and the liver-specific sodium/bile acid cotransporter, represented by this protein, that is found in the basolateral membranes of hepatocytes. Bile acids are the catabolic product of cholesterol metabolism, hence this protein is important for cholesterol homeostasis. [provided by RefSeq, Oct 2011]

Uniprot Description

NTCP: The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium. Belongs to the bile acid:sodium symporter (BASS) (TC 2.A.28) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: integral to plasma membrane; basolateral plasma membrane; plasma membrane

Molecular Function: bile acid:sodium symporter activity

Biological Process: bile acid and bile salt transport; bile acid metabolic process; transport; sodium ion transport; transmembrane transport

Research Articles on SLC10A1

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Product Notes

The Human SLC10A1 slc10a1 (Catalog #AAA2802043) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2802043 ELISA Kit recognizes Human SLC10A1. It is sometimes possible for the material contained within the vial of "Sodium/bile acid cotransporter (SLC10A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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