Typical Testing Data/Standard Curve (for reference only)
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Human Serine/arginine repetitive matrix protein 1 (SRRM1) ELISA Kit | SRRM1 elisa kit
Human Serine/arginine repetitive matrix protein 1 (SRRM1) ELISA Kit
Gene Names
SRRM1; 160-KD; POP101; SRM160
Reactivity
Human
Synonyms
Serine/arginine repetitive matrix protein 1 (SRRM1); Human Serine/arginine repetitive matrix protein 1 (SRRM1) ELISA Kit; SRRM1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SRRM1. No significant cross-reactivity or interference between SRRM1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SRRM1 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for SRRM1 elisa kit
Intended Uses: This SRRM1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SRRM1. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SRRM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SRRM1 antibody and an SRRM1-HRP conjugate. The assay sample and buffer are incubated together with SRRM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRRM1 concentration since SRRM1 from samples and SRRM1-HRP conjugate compete for the anti-SRRM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRRM1 from the sample, fewer sites are left to bind SRRM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRRM1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SRRM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-SRRM1 antibody and an SRRM1-HRP conjugate. The assay sample and buffer are incubated together with SRRM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRRM1 concentration since SRRM1 from samples and SRRM1-HRP conjugate compete for the anti-SRRM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SRRM1 from the sample, fewer sites are left to bind SRRM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRRM1 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for SRRM1 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102,126 Da
NCBI Official Full Name
serine/arginine repetitive matrix protein 1
NCBI Official Synonym Full Names
serine/arginine repetitive matrix 1
NCBI Official Symbol
SRRM1
NCBI Official Synonym Symbols
160-KD; POP101; SRM160
NCBI Protein Information
serine/arginine repetitive matrix protein 1; Ser/Arg-related nuclear matrix protein; SR-related nuclear matrix protein of 160 kDa
UniProt Protein Name
Serine/arginine repetitive matrix protein 1
Protein Family
UniProt Gene Name
SRRM1
UniProt Synonym Gene Names
SRM160; SRm160
UniProt Entry Name
SRRM1_HUMAN
Uniprot Description
SRm160: a Ser/Arg-related nuclear matrix protein. A splicing coactivator and component of the exon junction complex (EJC) involved in RNA export. Associates with proto-oncoprotein FUS on the nuclear matrix. Plays a role in spliceosome assembly.
Protein type: RNA-binding; RNA splicing; Spliceosome
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: nucleoplasm; nuclear matrix; nuclear speck; cytosol; nucleus
Molecular Function: DNA binding
Biological Process: transcription from RNA polymerase II promoter; RNA splicing, via transesterification reactions; nuclear mRNA splicing, via spliceosome; mRNA export from nucleus; RNA splicing; gene expression; mRNA 3'-end processing; termination of RNA polymerase II transcription
Research Articles on SRRM1
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Product Notes
The Human SRRM1 srrm1 (Catalog #AAA7249770) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7249770 ELISA Kit recognizes Human SRRM1. It is sometimes possible for the material contained within the vial of "Serine/arginine repetitive matrix protein 1 (SRRM1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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