Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Secretogranin-1 (CHGB/SCG1) Standard Curve.)
Human Secretogranin-1 (CHGB/SCG1) ELISA Kit | CHGB/SCG1 elisa kit
Human Secretogranin-1 (CHGB/SCG1) ELISA Kit
Reactivity
Human
Synonyms
Secretogranin-1 (CHGB/SCG1); Human Secretogranin-1 (CHGB/SCG1) ELISA Kit; CHGB; SCG1; chromogranin B; secretogranin B; CHGB/SCG1 elisa kit
Reactivity
Human
Specificity
Human Secretogranin-1 (CHGB/SCG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CHGB. No significant cross-reactivity or interference between Human CHGB and analogues was observed.
Sequence Length
646
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Secretogranin-1 (CHGB/SCG1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Secretogranin-1 (CHGB/SCG1) Standard Curve.)
Related Product Information for CHGB/SCG1 elisa kit
Description: This Human Secretogranin-1 (CHGB/SCG1) ELISA Kit employs a two-site sandwich ELISA to quantitate CHGB in samples. An antibody specific for CHGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHGB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHGB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Chromogranin B is a tyrosine-sulfated secretory protein found in a wide variety of peptidergic endocrine cells. Chromogranin functions as a neuroendocrine secretory granule protein which may be the precursor for other biologically active peptides.Secretoneurin is involved in chemotaxis of monocytes and eosinophils (comparable in potency to IL8) and endothelial cells and in regulation of endothelial cell proliferation.Highest secretoneurin levels are found in anterior pituitary, followed by adrenal medulla and posterior pituitary hypothalamus (2- to 6-fold lower levels in other brain regions investigated). Secretoneurin has been shown to have potent angiogenic activity in vivo in mouse cornea model and in vitro in a 3-dimensional gel.
Background: Chromogranin B is a tyrosine-sulfated secretory protein found in a wide variety of peptidergic endocrine cells. Chromogranin functions as a neuroendocrine secretory granule protein which may be the precursor for other biologically active peptides.Secretoneurin is involved in chemotaxis of monocytes and eosinophils (comparable in potency to IL8) and endothelial cells and in regulation of endothelial cell proliferation.Highest secretoneurin levels are found in anterior pituitary, followed by adrenal medulla and posterior pituitary hypothalamus (2- to 6-fold lower levels in other brain regions investigated). Secretoneurin has been shown to have potent angiogenic activity in vivo in mouse cornea model and in vitro in a 3-dimensional gel.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
secretogranin-1
NCBI Official Synonym Full Names
chromogranin B
NCBI Official Symbol
CHGB
NCBI Protein Information
secretogranin-1
UniProt Protein Name
Secretogranin-1
UniProt Gene Name
CHGB
UniProt Synonym Gene Names
CgB; SgI
UniProt Entry Name
SCG1_BOVIN
Uniprot Description
Function: Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides. The 16 pairs of basic AA distributed throughout its sequence may be used as proteolytic cleavage sites.
Research Articles on CHGB/SCG1
Similar Products
Product Notes
The Human CHGB/SCG1 chgb (Catalog #AAA9716258) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9716258 ELISA Kit recognizes Human CHGB/SCG1. It is sometimes possible for the material contained within the vial of "Secretogranin-1 (CHGB/SCG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
