Human Scavenger Receptor Class B Member 1 (SCARB1) ELISA Kit | SCARB1 elisa kit

Human Scavenger Receptor Class B Member 1 (SCARB1) ELISA Kit

Cat. Num. AAA2706345

• Gene Names: Scarb1; Srb1; SR-B1; Cd36l1
• Reactivity: Human
• Synonyms: Scavenger Receptor Class B Member 1 (SCARB1); Human Scavenger Receptor Class B Member 1 (SCARB1) ELISA Kit; CD36L1; SRB1; CLA1; SR-BI; Collagen Type I Receptor Thrombospondin Receptor-Like 1; CD36 and LIMPII analogous 1; SCARB1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Scavenger Receptor Class B Member 1 (SCARB1).; No significant cross-reactivity or interference between Scavenger Receptor Class B Member 1 (SCARB1) and analogues was observed.
• Sequence Length: 509

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 14.5pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Scavenger Receptor Class B Member 1 (SCARB1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Scavenger Receptor Class B Member 1 (SCARB1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SCARB1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Scavenger Receptor Class B Member 1 (SCARB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Scavenger Receptor Class B Member 1 (SCARB1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Scavenger Receptor Class B Member 1 (SCARB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Scavenger Receptor Class B Member 1 (SCARB1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13928730
• NCBI GeneID: 25073
• NCBI Accession #: NP_113729.1
• NCBI GenBank Nucleotide #: NM_031541.1
• UniProt Accession #: P97943
• Molecular Weight: 56,957 Da
• NCBI Official Full Name: scavenger receptor class B member 1
• NCBI Official Synonym Full Names: scavenger receptor class B, member 1
• NCBI Official Symbol: Scarb1
• NCBI Official Synonym Symbols: Srb1; SR-B1; Cd36l1
• NCBI Protein Information: scavenger receptor class B member 1
• UniProt Protein Name: Scavenger receptor class B member 1
• Protein Family: Scavenger receptor
• UniProt Gene Name: Scarb1
• UniProt Synonym Gene Names: SRB1

Uniprot Description

Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Receptor for HDL, mediating selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. Also facilitates the flux of free and esterified cholesterol between the cell surface and apoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. May be involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity.