Human S100 Calcium Binding Protein A5 (S100A5) ELISA Kit | S100A5 elisa kit

Human S100 Calcium Binding Protein A5 (S100A5) ELISA Kit

Cat. Num. AAA450891

• Gene Names: S100A5; S100D
• Reactivity: Human
• Synonyms: S100 Calcium Binding Protein A5 (S100A5); Human S100 Calcium Binding Protein A5 (S100A5) ELISA Kit; Probable RNA-binding protein 20; S100A5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of S100A5. No significant cross-reactivity or interference between S100A5 and analogues was observed.
• Sequence Length: 92

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.085ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100A5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100A5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for S100A5 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of S100A5 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to S100A5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to S100A5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain S100A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of S100A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 302632535
• NCBI GeneID: 6276
• NCBI Accession #: NP_002953.2
• NCBI GenBank Nucleotide #: NM_002962.1
• UniProt Accession #: P33763; Q52LE7; Q5RHS3
• Molecular Weight: 12,813 Da
• NCBI Official Full Name: protein S100-A5
• NCBI Official Synonym Full Names: S100 calcium binding protein A5
• NCBI Official Symbol: S100A5
• NCBI Official Synonym Symbols: S100D
• NCBI Protein Information: protein S100-A5
• UniProt Protein Name: Protein S100-A5
• Protein Family: Protein
• UniProt Gene Name: S100A5
• UniProt Synonym Gene Names: S100D

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. This protein is expressed in very restricted regions of the adult brain. [provided by RefSeq, Jul 2008]

Uniprot Description

Binds calcium, zinc and copper. One subunit can simultaneously bind 2 calcium ions or 2 copper ions plus 1 zinc ion. Calcium and copper ions compete for the same binding sites.