Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ReverseTri-iodothyronine (RT3) Standard Curve.)
Human Reverse Tri-iodothyronine (RT3) ELISA Kit | RT3 elisa kit
Human Reverse Tri-iodothyronine (RT3) ELISA Kit
Reactivity
Human
Synonyms
Reverse Tri-iodothyronine (RT3); Human Reverse Tri-iodothyronine (RT3) ELISA Kit; RT3 elisa kit
Reactivity
Human
Specificity
Human ReverseTri-iodothyronine (RT3) ELISA Kit has high sensitivity and excellent specificity for detection of Human RT3. No significant cross-reactivity or interference between Human RT3 and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ReverseTri-iodothyronine (RT3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human ReverseTri-iodothyronine (RT3) Standard Curve.)
Related Product Information for RT3 elisa kit
Description: This Human ReverseTri-iodothyronine (RT3) ELISA Kit employs a two-site sandwich ELISA to quantitate RT3 in samples. An antibody specific for RT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Reverse triiodothyronine is a molecule which is an isomer of triiodothyronine (T3). It is derived from thyroxine (T4) through the action of deiodinase.rT3, unlike T3, does not stimulate thyroid hormone receptors. However, rT3 nonetheless binds to these receptors, thereby blocking the action of T3. Under stress conditions, the adrenal glands produce excess amounts of cortisol. Cortisol inhibits the conversion of T4 to T3, thus shunting T4 conversion from T3 towards rT3. Consequently, there is a widespread shutdown in T3 binding across the body. This condition is termed Reverse T3 Dominance. It results in reduced body temperature, which slows the action of many enzymes, leading to a clinical syndrome, Multiple Enzyme Dysfunction, which produces the effects seen in hypothyroidism. Effects include fatigue, headache, migraine, PMS, irritability, fluid retention, anxiety and panic.
Background: Reverse triiodothyronine is a molecule which is an isomer of triiodothyronine (T3). It is derived from thyroxine (T4) through the action of deiodinase.rT3, unlike T3, does not stimulate thyroid hormone receptors. However, rT3 nonetheless binds to these receptors, thereby blocking the action of T3. Under stress conditions, the adrenal glands produce excess amounts of cortisol. Cortisol inhibits the conversion of T4 to T3, thus shunting T4 conversion from T3 towards rT3. Consequently, there is a widespread shutdown in T3 binding across the body. This condition is termed Reverse T3 Dominance. It results in reduced body temperature, which slows the action of many enzymes, leading to a clinical syndrome, Multiple Enzyme Dysfunction, which produces the effects seen in hypothyroidism. Effects include fatigue, headache, migraine, PMS, irritability, fluid retention, anxiety and panic.
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Product Notes
The Human RT3 (Catalog #AAA9714445) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714445 ELISA Kit recognizes Human RT3. It is sometimes possible for the material contained within the vial of "Reverse Tri-iodothyronine (RT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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