Human Retinoschisin (RS) ELISA Kit | RS elisa kit

Human Retinoschisin (RS) ELISA Kit

Cat. Num. AAA2021181

• Gene Names: RS1; RS; XLRS1
• Reactivity: Human
• Synonyms: Retinoschisin (RS); Human Retinoschisin (RS) ELISA Kit; RS1; XLRS1; Retinoschisis X-Linked; Juvenile 1; X-linked juvenile retinoschisis protein; RS elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Retinoschisin (RS).; No significant cross-reactivity or interference between Retinoschisin (RS) and analogues was observed.
• Sequence Length: 224

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoschisin (RS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoschisin (RS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoschisin (RS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoschisin (RS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoschisin (RS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoschisin (RS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RS elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 10835083
• NCBI GeneID: 6247
• NCBI Accession #: NP_000321.1
• NCBI GenBank Nucleotide #: NM_000330.3
• UniProt Accession #: O15537; Q0QD39
• Molecular Weight: 25,592 Da
• NCBI Official Full Name: retinoschisin
• NCBI Official Synonym Full Names: retinoschisin 1
• NCBI Official Symbol: RS1
• NCBI Official Synonym Symbols: RS; XLRS1
• NCBI Protein Information: retinoschisin
• UniProt Protein Name: Retinoschisin
• UniProt Gene Name: RS1
• UniProt Synonym Gene Names: XLRS1
• UniProt Entry Name: XLRS1_HUMAN

NCBI Description

This gene encodes an extracellular protein that plays a crucial role in the cellular organization of the retina. The encoded protein is assembled and secreted from photoreceptors and bipolar cells as a homo-oligomeric protein complex. Mutations in this gene are responsible for X-linked retinoschisis, a common, early-onset macular degeneration in males that results in a splitting of the inner layers of the retina and severe loss in vision. [provided by RefSeq, Oct 2008]

Uniprot Description

RS1: a highly conserved extracellular protein essential for proper retinal structure. Must interact with L-type voltage-gated calcium channels (L-VGCCs) correctly so that the photoreceptor cells in the eye can function properly. Genetic mutations in RS1 cause X-linked retinoschisis (XLRS) and early onset of macular degeneration. Interacts with the L-VGCCa1D subunit, regulating its activity. The expression and secretion of retinoschisin are modulated by circadian rhythms, peaking at night and diminishing during the day.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xp22.13

Cellular Component: extrinsic to plasma membrane; extracellular space

Molecular Function: phosphatidylinositol-4,5-bisphosphate binding; phosphatidylinositol-3,4,5-triphosphate binding; phosphatidylserine binding; phosphatidylinositol-5-phosphate binding; phosphatidylinositol-3,4-bisphosphate binding; phosphatidylinositol 3-phosphate binding

Biological Process: visual perception; multicellular organismal development; adaptation of rhodopsin mediated signaling; cell adhesion

Disease: Retinoschisis 1, X-linked, Juvenile