Human Retinol Binding Protein 3, Interstitial (RBP3) ELISA Kit | RBP3 elisa kit

Human Retinol Binding Protein 3, Interstitial (RBP3) ELISA Kit

Cat. Num. AAA2024501

• Gene Names: RBP3; IRBP; RBPI; RP66; D10S64; D10S65; D10S66
• Reactivity: Human
• Synonyms: Retinol Binding Protein 3; Interstitial (RBP3); Human Retinol Binding Protein 3; Interstitial (RBP3) ELISA Kit; RBP-3; IRBP; RBPI; Interphotoreceptor retinoid-binding protein; Interstitial retinol-binding protein; RBP3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Retinol Binding Protein 3, Interstitial (RBP3).; No significant cross-reactivity or interference between Retinol Binding Protein 3, Interstitial (RBP3) and analogues was observed.
• Sequence Length: 1,247

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 3, Interstitial (RBP3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 3, Interstitial (RBP3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 3, Interstitial (RBP3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 3, Interstitial (RBP3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinol Binding Protein 3, Interstitial (RBP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinol Binding Protein 3, Interstitial (RBP3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RBP3 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119571044
• NCBI GeneID: 5949
• UniProt Accession #: P10745; Q0QD34; Q5VSR0; Q8IXN0
• Molecular Weight: 135,363 Da
• NCBI Official Full Name: retinol binding protein 3, interstitial
• NCBI Official Synonym Full Names: retinol binding protein 3, interstitial
• NCBI Official Symbol: RBP3
• NCBI Official Synonym Symbols: IRBP; RBPI; RP66; D10S64; D10S65; D10S66
• NCBI Protein Information: retinol-binding protein 3
• UniProt Protein Name: Retinol-binding protein 3
• Protein Family: Retinol-binding protein
• UniProt Gene Name: RBP3
• UniProt Synonym Gene Names: IRBP
• UniProt Entry Name: RET3_HUMAN

NCBI Description

Interphotoreceptor retinol-binding protein is a large glycoprotein known to bind retinoids and found primarily in the interphotoreceptor matrix of the retina between the retinal pigment epithelium and the photoreceptor cells. It is thought to transport retinoids between the retinal pigment epithelium and the photoreceptors, a critical role in the visual process.The human IRBP gene is approximately 9.5 kbp in length and consists of four exons separated by three introns. The introns are 1.6-1.9 kbp long. The gene is transcribed by photoreceptor and retinoblastoma cells into an approximately 4.3-kilobase mRNA that is translated and processed into a glycosylated protein of 135,000 Da. The amino acid sequence of human IRBP can be divided into four contiguous homology domains with 33-38% identity, suggesting a series of gene duplication events. In the gene, the boundaries of these domains are not defined by exon-intron junctions, as might have been expected. The first three homology domains and part of the fourth are all encoded by the first large exon, which is 3,180 base pairs long. The remainder of the fourth domain is encoded in the last three exons, which are 191, 143, and approximately 740 base pairs long, respectively. [provided by RefSeq, Jul 2008]

Uniprot Description

RBP3: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. Belongs to the peptidase S41A family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; extracellular region; vesicle

Molecular Function: retinoid binding; serine-type peptidase activity; retinal binding; retinol binding

Biological Process: phototransduction, visible light; visual perception; transport; lipid metabolic process; retinoid metabolic process; proteolysis

Disease: Retinitis Pigmentosa 66; Retinitis Pigmentosa