Human Retinoic Acid Receptor Alpha (RARa) ELISA Kit | RARa elisa kit

Human Retinoic Acid Receptor Alpha (RARa) ELISA Kit

Cat. Num. AAA2702768

• Gene Names: RARA; RAR; NR1B1
• Reactivity: Human
• Synonyms: Retinoic Acid Receptor Alpha (RARa); Human Retinoic Acid Receptor Alpha (RARa) ELISA Kit; RAR-A; NR1B1; RAR; RAR-Alpha; Nuclear Receptor Subfamily 1 Group B Member 1; RARa elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Retinoic Acid Receptor Alpha (RARa).; No significant cross-reactivity or interference between Retinoic Acid Receptor Alpha (RARa) and analogues was observed.
• Sequence Length: 365

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoic Acid Receptor Alpha (RARa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RARa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoic Acid Receptor Alpha (RARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoic Acid Receptor Alpha (RARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoic Acid Receptor Alpha (RARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Retinoic Acid Receptor Alpha (RARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8815561
• NCBI GeneID: 5914
• UniProt Accession #: P10276; P78456; Q13440; Q13441; Q96S41; Q9NQS0; B8Y636
• Molecular Weight: 39,700 Da
• NCBI Official Full Name: retinoic acid receptor alpha, partial
• NCBI Official Synonym Full Names: retinoic acid receptor alpha
• NCBI Official Symbol: RARA
• NCBI Official Synonym Symbols: RAR; NR1B1
• NCBI Protein Information: retinoic acid receptor alpha
• UniProt Protein Name: Retinoic acid receptor alpha
• Protein Family: Retinoic acid receptor
• UniProt Gene Name: RARA
• UniProt Synonym Gene Names: NR1B1; RAR-alpha

NCBI Description

This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.[provided by RefSeq, Sep 2010]

Uniprot Description

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function (). Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.