Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) Standard Curve.)
Human Regenerating Islet-Derived 1 Alpha/Pancreatic Stone Protein/Pancreatic Thread Protein (REG1A) ELISA Kit | REG1A elisa kit
Human Regenerating Islet-Derived 1 Alpha/Pancreatic Stone Protein/Pancreatic Thread Protein (REG1A) ELISA Kit
Gene Names
REG1A; P19; PSP; PTP; REG; ICRF; PSPS; PSPS1
Reactivity
Human
Synonyms
Regenerating Islet-Derived 1 Alpha/Pancreatic Stone Protein/Pancreatic Thread Protein (REG1A); Human Regenerating Islet-Derived 1 Alpha/Pancreatic Stone Protein/Pancreatic Thread Protein (REG1A) ELISA Kit; REG1A; ICRF; MGC12447; P19; PSP; PSPS; PSPS1; PTP; REG; islet cells regeneration factor; islet of langerhans regenerating protein; lithostathine 1 alpha; pancreatic stone protein; secretory; pancreatic thread; REG1A elisa kit
Reactivity
Human
Specificity
Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human REG1A. No significant cross-reactivity or interference between Human REG1A and analogues was observed.
Sequence Length
166
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) Standard Curve.)
Related Product Information for REG1A elisa kit
Description: This Human Regenerating Islet-derived 1 Alpha/pancreatic Stone Protein/pancreatic Thread Protein (REG1A) ELISA Kit employs a two-site sandwich ELISA to quantitate REG1A in samples. An antibody specific for REG1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Pancreatic stone protein is the major component of the protein matrix of calculi in patients suffering from chronic calcifying pancreatitis. Secretory pancreatic stone protein is a glycoprotein in the pancreatic secretion. This protein, called PSPS, occurs in multiple molecular forms due to posttranslational processing. The abundance of PSPS in pancreatic juice (10 to 14% of total protein) suggests that it plays an important role in exocrine pancreatic function. In vitro experiments show that PSPS inhibits CaCO(3) crystal growth. Since in all the pancreatic secretions are supersaturated in calcium carbonate, the physiologic role of PSPS may be related to its inhibitory properties. This gene encodes a protein that is secreted by the exocrine pancreas.
Background: Pancreatic stone protein is the major component of the protein matrix of calculi in patients suffering from chronic calcifying pancreatitis. Secretory pancreatic stone protein is a glycoprotein in the pancreatic secretion. This protein, called PSPS, occurs in multiple molecular forms due to posttranslational processing. The abundance of PSPS in pancreatic juice (10 to 14% of total protein) suggests that it plays an important role in exocrine pancreatic function. In vitro experiments show that PSPS inhibits CaCO(3) crystal growth. Since in all the pancreatic secretions are supersaturated in calcium carbonate, the physiologic role of PSPS may be related to its inhibitory properties. This gene encodes a protein that is secreted by the exocrine pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Lithostathine-1-alpha
NCBI Official Synonym Full Names
regenerating family member 1 alpha
NCBI Official Symbol
REG1A
NCBI Official Synonym Symbols
P19; PSP; PTP; REG; ICRF; PSPS; PSPS1
NCBI Protein Information
lithostathine-1-alpha
UniProt Protein Name
Lithostathine-1-alpha
Protein Family
UniProt Gene Name
REG1A
UniProt Synonym Gene Names
PSPS; PSPS1; REG; ICRF; REG; PSP; PTP; REG-1-alpha
UniProt Entry Name
REG1A_HUMAN
NCBI Description
This gene is a type I subclass member of the Reg gene family. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. [provided by RefSeq, Jul 2008]
Uniprot Description
REG1A: Might act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 2p12
Molecular Function: growth factor activity; carbohydrate binding
Biological Process: positive regulation of cell proliferation
Research Articles on REG1A
Similar Products
Product Notes
The Human REG1A reg1a (Catalog #AAA9714479) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714479 ELISA Kit recognizes Human REG1A. It is sometimes possible for the material contained within the vial of "Regenerating Islet-Derived 1 Alpha/Pancreatic Stone Protein/Pancreatic Thread Protein (REG1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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