Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human RCC1 and BTB domain-containing protein 2 (RCBTB2) Standard Curve.)
Human RCC1 And BTB Domain-Containing Protein 2 (RCBTB2) ELISA Kit | RCBTB2 elisa kit
Human RCC1 And BTB Domain-Containing Protein 2 (RCBTB2) ELISA Kit
Gene Names
RCBTB2; RLG; CHC1L
Reactivity
Human
Synonyms
RCC1 And BTB Domain-Containing Protein 2 (RCBTB2); Human RCC1 And BTB Domain-Containing Protein 2 (RCBTB2) ELISA Kit; RCBTB2; CHC1L; RCC1-like G exchanging factor RLG; chromosome condensation 1-like; regulator of chromosome condensation and BTB domain containing protein 2; RCBTB2 elisa kit
Reactivity
Human
Specificity
Human RCC1 and BTB domain-containing protein 2 (RCBTB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human RCBTB2. No significant cross-reactivity or interference between Human RCBTB2 and analogues was observed.
Sequence Length
551
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human RCC1 and BTB domain-containing protein 2 (RCBTB2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human RCC1 and BTB domain-containing protein 2 (RCBTB2) Standard Curve.)
Related Product Information for RCBTB2 elisa kit
Description: This Human RCC1 and BTB domain-containing protein 2 (RCBTB2) ELISA Kit employs a two-site sandwich ELISA to quantitate RCBTB2 in samples. An antibody specific for RCBTB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RCBTB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RCBTB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RCBTB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RCBTB2 encodes a member of the RCC1-related GEF family. The N-terminal half of the encoded amino acid sequence shows similarity to the regulator of chromosome condensation RCC1, which acts as a guanine nucleotide exchange factor (GEF) protein for the Ras-related GTPase Ran. The CHC1L gene contains 14 exons and spans at least 30 kb. The 3.2-kb CHC1L mRNA was expressed in all tissues tested by Northern blot analysis. The authors identified 4 alternatively spliced CHC1L transcripts encoding 2 protein isoforms of 551 and 526 amino acids, respectively. Sequence analysis revealed that both isoforms contain 6 repeats that share homology with repeats found in CHC1 and RPGR, and a zinc finger motif.
Background: RCBTB2 encodes a member of the RCC1-related GEF family. The N-terminal half of the encoded amino acid sequence shows similarity to the regulator of chromosome condensation RCC1, which acts as a guanine nucleotide exchange factor (GEF) protein for the Ras-related GTPase Ran. The CHC1L gene contains 14 exons and spans at least 30 kb. The 3.2-kb CHC1L mRNA was expressed in all tissues tested by Northern blot analysis. The authors identified 4 alternatively spliced CHC1L transcripts encoding 2 protein isoforms of 551 and 526 amino acids, respectively. Sequence analysis revealed that both isoforms contain 6 repeats that share homology with repeats found in CHC1 and RPGR, and a zinc finger motif.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
RCC1 and BTB domain-containing protein 2 isoform 2
NCBI Official Synonym Full Names
RCC1 and BTB domain containing protein 2
NCBI Official Symbol
RCBTB2
NCBI Official Synonym Symbols
RLG; CHC1L
NCBI Protein Information
RCC1 and BTB domain-containing protein 2
UniProt Protein Name
RCC1 and BTB domain-containing protein 2
Protein Family
UniProt Gene Name
RCBTB2
UniProt Synonym Gene Names
CHC1L; RLG; CHC1-L
UniProt Entry Name
RCBT2_HUMAN
NCBI Description
This gene encodes a protein containing two C-terminal BTB/POZ domains that is related to regulator of chromosome condensation (RCC). The encoded protein may act as a guanine nucleotide exchange factor. This gene is observed to be lost or underexpressed in prostate cancers. There is a pseudogene of this gene on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
Research Articles on RCBTB2
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Product Notes
The Human RCBTB2 rcbtb2 (Catalog #AAA9714481) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714481 ELISA Kit recognizes Human RCBTB2. It is sometimes possible for the material contained within the vial of "RCC1 And BTB Domain-Containing Protein 2 (RCBTB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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