Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) Standard Curve.)
Human Pyridoxal/Pyridoxine Vitamin B6-Kinase (PDXK) ELISA Kit | PDXK elisa kit
Human Pyridoxal/Pyridoxine Vitamin B6-Kinase (PDXK) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pyridoxal/pyridoxine vitamin B6-kinase (PDXK) Standard Curve.)
Background: Pyridoxal (pyridoxine, vitamin B6) kinase is also known as PDXK. The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Hanna et al. (1997) cloned the cDNA encoding PDXK, which they referred to as PKH. The PDXK gene encodes a 312-amino acid polypeptide, and expression of the cDNA revealed pyridoxal kinase activity. Northern blot analysis revealed that a major 1.5-kb PDXK transcript was expressed in all tissues tested.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene phosphorylates vitamin B6, a step required for the conversion of vitamin B6 to pyridoxal-5-phosphate, an important cofactor in intermediary metabolism. The encoded protein is cytoplasmic and probably acts as a homodimer. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
PDXK: Required for synthesis of pyridoxal-5-phosphate from vitamin B6. Belongs to the pyridoxine kinase family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.1.35; Cofactor and Vitamin Metabolism - vitamin B6; Kinase, other
Chromosomal Location of Human Ortholog: 21q22.3
Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus
Molecular Function: potassium ion binding; sodium ion binding; protein homodimerization activity; zinc ion binding; pyridoxal kinase activity; magnesium ion binding; lithium ion binding; ATP binding; pyridoxal phosphate binding
Biological Process: cell proliferation; vitamin metabolic process; vitamin B6 metabolic process; pyridoxal 5'-phosphate salvage; phosphorylation; water-soluble vitamin metabolic process; pyridoxal phosphate biosynthetic process
Research Articles on PDXK
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Product Notes
The Human PDXK pdxk (Catalog #AAA9714923) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714923 ELISA Kit recognizes Human PDXK. It is sometimes possible for the material contained within the vial of "Pyridoxal/Pyridoxine Vitamin B6-Kinase (PDXK), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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