Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pygopus homolog 1 (PYGO1) Standard Curve.)
Human Pygopus Homolog 1 (PYGO1) ELISA Kit | PYGO1 elisa kit
Human Pygopus Homolog 1 (PYGO1) ELISA Kit
Reactivity
Human
Synonyms
Pygopus Homolog 1 (PYGO1); Human Pygopus Homolog 1 (PYGO1) ELISA Kit; PYGO1; DKFZp547G0910; pygopus 1; pygopus homolog 1; pygopus-like protein 1; PYGO1 elisa kit
Reactivity
Human
Specificity
Human Pygopus homolog 1 (PYGO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PYGO1. No significant cross-reactivity or interference between Human PYGO1 and analogues was observed.
Sequence Length
419
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pygopus homolog 1 (PYGO1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Pygopus homolog 1 (PYGO1) Standard Curve.)
Related Product Information for PYGO1 elisa kit
Description: This Human Pygopus homolog 1 (PYGO1) ELISA Kit employs a two-site sandwich ELISA to quantitate PYGO1 in samples. An antibody specific for PYGO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PYGO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PYGO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PYGO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: WNT signaling controls many fundamental processes during animal development. WNT transduction is mediated by the association of beta-catenin with nuclear TCF DNA-binding factors.Lgs encodes the homolog of human BCL9, and the authors provided genetic and molecular evidence that these proteins exert their function by physically linking Pygo to beta-catenin. The PYGO1 and PYGO2 proteins, which contain 419 and 406 amino acids, respectively, possess a highly conserved PHD finger that interacts with homology domain-1 (HD1) of BCL9. The findings suggested that the recruitment of PYGO permits beta-catenin to transcriptionally activate WNT target genes and raised the possibility that a deregulation of these events may play a causal role in the development of B-cell malignancies.
Background: WNT signaling controls many fundamental processes during animal development. WNT transduction is mediated by the association of beta-catenin with nuclear TCF DNA-binding factors.Lgs encodes the homolog of human BCL9, and the authors provided genetic and molecular evidence that these proteins exert their function by physically linking Pygo to beta-catenin. The PYGO1 and PYGO2 proteins, which contain 419 and 406 amino acids, respectively, possess a highly conserved PHD finger that interacts with homology domain-1 (HD1) of BCL9. The findings suggested that the recruitment of PYGO permits beta-catenin to transcriptionally activate WNT target genes and raised the possibility that a deregulation of these events may play a causal role in the development of B-cell malignancies.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
pygopus homolog 1 isoform 1
NCBI Official Synonym Full Names
pygopus family PHD finger 1
NCBI Official Symbol
PYGO1
NCBI Protein Information
pygopus homolog 1
UniProt Protein Name
Pygopus homolog 1
Protein Family
UniProt Gene Name
PYGO1
UniProt Entry Name
PYGO1_HUMAN
Uniprot Description
Function: Involved in signal transduction through the Wnt pathway.
Subunit structure: Binds to BCL9 via The PHD-type zinc finger motiv, and thereby becomes part of the nuclear beta-catenin/TCF complex. Found in a complex with BCL9L, CDC73, CTNNB1 and PYGO1.
Subcellular location: Nucleus
Probable.
Sequence similarities: Contains 1 PHD-type zinc finger.
Research Articles on PYGO1
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Product Notes
The Human PYGO1 pygo1 (Catalog #AAA9714680) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714680 ELISA Kit recognizes Human PYGO1. It is sometimes possible for the material contained within the vial of "Pygopus Homolog 1 (PYGO1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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