Human Putative palmitoyltransferase ZDHHC22 ELISA Kit | ZDHHC22 elisa kit
Human Putative palmitoyltransferase ZDHHC22 (ZDHHC22) ELISA Kit
Gene Names
ZDHHC22; C14orf59
Reactivity
Human
Synonyms
Putative palmitoyltransferase ZDHHC22; Human Putative palmitoyltransferase ZDHHC22 (ZDHHC22) ELISA Kit; ZDHHC22 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ZDHHC22. No significant cross-reactivity or interference between Human ZDHHC22 and analogues was observed.
Sequence Length
263
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for ZDHHC22 elisa kit
ZDHHC22 belongs to the DHHC palmitoyltransferase family.Contains 1 DHHC-type zinc finger.The DHHC domain is required for palmitoyltransferase activity.
In molecular biology the DHHC domain is a protein domain that acts as an enzyme, which adds a palmitoyl chemical group to proteins in order to anchor them to cell membranes. The DHHC domain was discovered in 1999 and named after a conserved sequence motif found in its protein sequence. Roth and colleagues showed that the yeast Akr1p protein could palmitoylate Yck2p in vitro and inferred that the DHHC domain defined a large family of palmitoyltransferases. In mammals twenty three members of this family have been identified and their substrate specificities investigated.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZDHHC22 in samples. An antibody specific for ZDHHC22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZDHHC22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZDHHC22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZDHHC22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
In molecular biology the DHHC domain is a protein domain that acts as an enzyme, which adds a palmitoyl chemical group to proteins in order to anchor them to cell membranes. The DHHC domain was discovered in 1999 and named after a conserved sequence motif found in its protein sequence. Roth and colleagues showed that the yeast Akr1p protein could palmitoylate Yck2p in vitro and inferred that the DHHC domain defined a large family of palmitoyltransferases. In mammals twenty three members of this family have been identified and their substrate specificities investigated.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ZDHHC22 in samples. An antibody specific for ZDHHC22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZDHHC22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZDHHC22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZDHHC22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,100 Da
NCBI Official Full Name
palmitoyltransferase ZDHHC22
NCBI Official Synonym Full Names
zinc finger DHHC-type containing 22
NCBI Official Symbol
ZDHHC22
NCBI Official Synonym Symbols
C14orf59
NCBI Protein Information
palmitoyltransferase ZDHHC22
UniProt Protein Name
Palmitoyltransferase ZDHHC22
Protein Family
UniProt Gene Name
ZDHHC22
UniProt Synonym Gene Names
C14orf59; DHHC-22; zDHHC22
Uniprot Description
ZDHHC22: Belongs to the DHHC palmitoyltransferase family.
Protein type: EC 2.3.1.225; Membrane protein, integral; Membrane protein, multi-pass; Transferase
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: endoplasmic reticulum; Golgi apparatus; integral component of membrane
Molecular Function: protein binding; protein-cysteine S-palmitoyltransferase activity
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Product Notes
The Human ZDHHC22 zdhhc22 (Catalog #AAA281192) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA281192 ELISA Kit recognizes Human ZDHHC22. It is sometimes possible for the material contained within the vial of "Putative palmitoyltransferase ZDHHC22, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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