Typical Testing Data/Standard Curve (for reference only)
Human Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) ELISA Kit | P2RY14 elisa kit
Human Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14) ELISA Kit
Gene Names
P2RY14; P2Y14; BPR105; GPR105
Reactivity
Human
Synonyms
Purinergic Receptor P2Y; G Protein Coupled 14 (P2RY14); Human Purinergic Receptor P2Y; G Protein Coupled 14 (P2RY14) ELISA Kit; P2RY14 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of P2RY14. No significant cross-reactivity or interference between P2RY14 and analogues was observed.
Sequence Length
338
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.053ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level P2RY14 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level P2RY14 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for P2RY14 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of P2RY14 in human tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P2RY14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to P2RY14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P2RY14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of P2RY14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to P2RY14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to P2RY14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain P2RY14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of P2RY14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,971 Da
NCBI Official Full Name
P2Y purinoceptor 14
NCBI Official Synonym Full Names
purinergic receptor P2Y14
NCBI Official Symbol
P2RY14
NCBI Official Synonym Symbols
P2Y14; BPR105; GPR105
NCBI Protein Information
P2Y purinoceptor 14
UniProt Protein Name
P2Y purinoceptor 14
Protein Family
UniProt Gene Name
P2RY14
UniProt Synonym Gene Names
GPR105; KIAA0001; P2Y14
NCBI Description
The product of this gene belongs to the family of G-protein coupled receptors, which contains several receptor subtypes with different pharmacological selectivity for various adenosine and uridine nucleotides. This receptor is a P2Y purinergic receptor for UDP-glucose and other UDP-sugars coupled to G-proteins. It has been implicated in extending the known immune system functions of P2Y receptors by participating in the regulation of the stem cell compartment, and it may also play a role in neuroimmune function. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor for UDP-glucose and other UDP-sugar coupled to G-proteins. Not activated by ATP, ADP, UTP or ATP.
Research Articles on P2RY14
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Product Notes
The Human P2RY14 p2ry14 (Catalog #AAA455329) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA455329 ELISA Kit recognizes Human P2RY14. It is sometimes possible for the material contained within the vial of "Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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