Human PSA ELISA Kit | PSA elisa kit
PSA (Total) ELISA
Principle of the Assay: The PSA ELISA test is a solid phase assay based on a streptavidin-biotin principle. The standards, samples and a reagent mixture of anti-PSA enzyme and biotin conjugates (conjugate reagent) are added into the wells that are coated with streptavidin. PSA in the serum forms a sandwich between two highly specific anti-PSA antibodies, labeled with biotin and HRP. Simultaneously, the biotinylated antibody is immobilized onto the wells through a high affinity streptavidin-biotin interaction. Unbound protein and excess biotin/enzyme conjugated reagent are washed off by washing buffer. Upon addition of the substrate, the intensity of the color developed is directly proportional to the concentration of PSA in the samples. A standard curve is prepared relating color intensity to the concentration of the PSA.
NCBI and Uniprot Product Information
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PSA: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. Forms heterodimer with SERPINA5. Inhibited by SERPINA5. Activity is strongly inhibited by Zn2+, 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins, which are avid zinc binders. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.21.77
Chromosomal Location of Human Ortholog: 19q13.41
Cellular Component: extracellular region; nucleus
Molecular Function: protein binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: negative regulation of angiogenesis; cellular protein metabolic process; proteolysis
Research Articles on PSA
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Product Notes
The Human PSA klk3 (Catalog #AAA494521) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA494521 ELISA Kit recognizes Human PSA. It is sometimes possible for the material contained within the vial of "PSA, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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