Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Prothymosin, alpha (PTMA) Standard Curve.)
Human Prothymosin, alpha (PTMA) ELISA Kit | PTMA elisa kit
Human Prothymosin, alpha (PTMA) ELISA Kit
Gene Names
PTMA; TMSA
Reactivity
Human
Synonyms
Prothymosin; alpha (PTMA); Human Prothymosin; alpha (PTMA) ELISA Kit; PTMA; MGC104802; TMSA; gene sequence 28; prothymosin alpha protein; PTMA elisa kit
Reactivity
Human
Specificity
Human Prothymosin, alpha (PTMA) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTMA. No significant cross-reactivity or interference between Human PTMA and analogues was observed.
Sequence Length
110
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Prothymosin, alpha (PTMA) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Prothymosin, alpha (PTMA) Standard Curve.)
Related Product Information for PTMA elisa kit
Description: This Human Prothymosin, alpha (PTMA) ELISA Kit employs a two-site sandwich ELISA to quantitate PTMA in samples. An antibody specific for PTMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTMA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTMA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prothymosin alpha is a protein encoded by the PTMA. PTMA is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin alpha-1. The encoded human PTMA protein is a highly acidic (54 residues out of 111) and shared over 90% sequence homology with rat PTMA. The primary structure of PTMA is highly conserved and shows a number of important features. The first 28 amino acids of its sequence correspond to those of thymosin-alpha-1. It has a central acidic region (residues 41-85) comprising of glutamic and aspartic residues. Neither type of secondary structure has been detected. Thymosin-alpha-1 appeared at positions 2-29 of the PTMA.PTMA has been shown to serve essential biological functions.
Background: Prothymosin alpha is a protein encoded by the PTMA. PTMA is a small, 12.4 kDa protein. It is a 109-111 amino acid long polypeptide as the precursor of thymosin alpha-1. The encoded human PTMA protein is a highly acidic (54 residues out of 111) and shared over 90% sequence homology with rat PTMA. The primary structure of PTMA is highly conserved and shows a number of important features. The first 28 amino acids of its sequence correspond to those of thymosin-alpha-1. It has a central acidic region (residues 41-85) comprising of glutamic and aspartic residues. Neither type of secondary structure has been detected. Thymosin-alpha-1 appeared at positions 2-29 of the PTMA.PTMA has been shown to serve essential biological functions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
Prothymosin, alpha
NCBI Official Synonym Full Names
prothymosin alpha
NCBI Official Symbol
PTMA
NCBI Official Synonym Symbols
TMSA
NCBI Protein Information
prothymosin alpha
Protein Family
Research Articles on PTMA
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Product Notes
The Human PTMA (Catalog #AAA9714724) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714724 ELISA Kit recognizes Human PTMA. It is sometimes possible for the material contained within the vial of "Prothymosin, alpha (PTMA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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