Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S100-A7A (S100A7A) Standard Curve.)
Human Protein S100-A7A (S100A7A) ELISA Kit | S100A7A elisa kit
Human Protein S100-A7A (S100A7A) ELISA Kit
Gene Names
S100A7A; NICE2; NICE-2; S100A15; S100A7f; S100A7L1
Reactivity
Human
Synonyms
Protein S100-A7A (S100A7A); Human Protein S100-A7A (S100A7A) ELISA Kit; S100A7A; NICE-2; S100A15; S100A7L1; S100A7f; OTTHUMP00000015325; S100 calcium binding protein A15; S100 calcium binding protein A7-like 1; S100A7A elisa kit
Reactivity
Human
Specificity
Human Protein S100-A7A (S100A7A) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A7A. No significant cross-reactivity or interference between Human S100A7A and analogues was observed.
Sequence Length
101
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S100-A7A (S100A7A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Protein S100-A7A (S100A7A) Standard Curve.)
Related Product Information for S100A7A elisa kit
Description: This Human Protein S100-A7A (S100A7A) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A7A in samples. An antibody specific for S100A7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A7A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A7A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The human calcium-binding protein (hS100A15) was first identified in inflamed hyperplastic psoriatic skin, where the S100A15 gene is transcribed into two mRNA splice variants, hS100A15-S and hS100A15-L. In cultured keratinocytes, IL-1beta and Th1 cytokines significantly induced hS100A15-L compared with hS100A15-S. In contrast, Th2-derived cytokines had no influence on the expression of either hS100A15 splice variant. Differentiation of human keratinocytes induced by 1.2 mm calcium resulted in the upregulation of both hS100A15 mRNA isoforms. Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin.
Background: The human calcium-binding protein (hS100A15) was first identified in inflamed hyperplastic psoriatic skin, where the S100A15 gene is transcribed into two mRNA splice variants, hS100A15-S and hS100A15-L. In cultured keratinocytes, IL-1beta and Th1 cytokines significantly induced hS100A15-L compared with hS100A15-S. In contrast, Th2-derived cytokines had no influence on the expression of either hS100A15 splice variant. Differentiation of human keratinocytes induced by 1.2 mm calcium resulted in the upregulation of both hS100A15 mRNA isoforms. Overexpression of hS100A15 in chronic inflammatory skin diseases and regulation by inflammatory cytokines and calcium suggest that hS100A15 is involved in Th1-associated epithelial responses and epidermal maturation in normal and diseased human skin.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein S100-A7A
NCBI Official Synonym Full Names
S100 calcium binding protein A7A
NCBI Official Symbol
S100A7A
NCBI Official Synonym Symbols
NICE2; NICE-2; S100A15; S100A7f; S100A7L1
NCBI Protein Information
protein S100-A7A
UniProt Protein Name
Protein S100-A7A
Protein Family
UniProt Gene Name
S100A7A
UniProt Synonym Gene Names
S100A15; S100A7L1
UniProt Entry Name
S1A7A_HUMAN
Uniprot Description
S100A7A: May be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases. Belongs to the S-100 family.
Protein type: Cell development/differentiation
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: cytoplasm
Molecular Function: calcium ion binding
Research Articles on S100A7A
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Product Notes
The Human S100A7A s100a7a (Catalog #AAA9714426) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714426 ELISA Kit recognizes Human S100A7A. It is sometimes possible for the material contained within the vial of "Protein S100-A7A (S100A7A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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