Human Protein eyes shut homolog (EYS) ELISA Kit | EYS elisa kit
Human Protein eyes shut homolog (EYS) ELISA Kit
Gene Names
EYS; RP25; SPAM; EGFL10; EGFL11; C6orf178; C6orf179; C6orf180; bA74E24.1; dJ22I17.2; bA166P24.2; bA307F22.3; dJ1018A4.2; dJ303F19.1
Reactivity
Human
Synonyms
Protein eyes shut homolog (EYS); Human Protein eyes shut homolog (EYS) ELISA Kit; RP1-22I17.2; C6orf178; C6orf179; C6orf180; EGFL10; EGFL11; KIAA0663; RP25; SPAM; bA166P24.2; bA307F22.3; bA74E24.1; dJ1018A4.2; dJ22I17.2; dJ303F19.1; EGF-like-domain; multiple 10; m; EYS elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human EYS. No significant cross-reactivity or interference between Human EYS and analogues was observed.
Sequence Length
3144
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
62.5 pg/mL - 4000 pg/mL
Sensitivity
31.25 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for EYS elisa kit
Intended Uses: For the quantitative detection of Human Protein eyes shut homolog (EYS) concentration in serum, plasma, tissue homogenates and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EYS in Human serum, plasma, tissue homogenates. An antibody specific for EYS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EYS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EYS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EYS bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate EYS in Human serum, plasma, tissue homogenates. An antibody specific for EYS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EYS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EYS is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EYS bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,877 Da
NCBI Official Full Name
protein eyes shut homolog isoform 1
NCBI Official Synonym Full Names
eyes shut homolog (Drosophila)
NCBI Official Symbol
EYS
NCBI Official Synonym Symbols
RP25; SPAM; EGFL10; EGFL11; C6orf178; C6orf179; C6orf180; bA74E24.1; dJ22I17.2; bA166P24.2; bA307F22.3; dJ1018A4.2; dJ303F19.1
NCBI Protein Information
protein eyes shut homolog
UniProt Protein Name
Protein eyes shut homolog
Protein Family
UniProt Gene Name
EYS
UniProt Synonym Gene Names
C6orf178; C6orf179; C6orf180; EGFL10; EGFL11; SPAM; EGF-like protein 10; EGF-like protein 11
NCBI Description
The product of this gene contains multiple epidermal growth factor (EGF)-like and LamG domains. The protein is expressed in the photoreceptor layer of the retina, and the gene is mutated in autosomal recessive retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
Uniprot Description
Required to maintain the integrity of photoreceptor cells.
Research Articles on EYS
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Product Notes
The Human EYS eys (Catalog #AAA287686) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA287686 ELISA Kit recognizes Human EYS. It is sometimes possible for the material contained within the vial of "Protein eyes shut homolog (EYS), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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