Human Proteasome Activator Subunit 1 ELISA Kit | PSME3 elisa kit

Human Proteasome Activator Subunit 1 ELISA Kit

Cat. Num. AAA3804598

• Gene Names: PSME3; Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
• Reactivity: Human
• Synonyms: Proteasome Activator Subunit 1; Human Proteasome Activator Subunit 1 ELISA Kit; PSME3 elisa kit

• Reactivity: Human
• Sequence Length: 254

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.625ng/ml-10ng/ml
• Sensitivity: 0.1 ng/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSME3 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofPROTEASOME ACTIVATOR SUBUNIT 1in the sample, This PROTEASOME ACTIVATOR SUBUNIT 1ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PROTEASOME ACTIVATOR SUBUNIT 1concentration. The concentration of PROTEASOME ACTIVATOR SUBUNIT 1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 30410794
• NCBI GeneID: 10197
• NCBI Accession #: NP_005780.2
• NCBI GenBank Nucleotide #: NP_005780.2
• UniProt Accession #: P61289
• NCBI Official Full Name: proteasome activator complex subunit 3 isoform 1
• NCBI Official Synonym Full Names: proteasome activator subunit 3
• NCBI Official Symbol: PSME3
• NCBI Official Synonym Symbols: Ki; PA28G; HEL-S-283; PA28gamma; REG-GAMMA; PA28-gamma
• NCBI Protein Information: proteasome activator complex subunit 3
• UniProt Protein Name: Proteasome activator complex subunit 3
• Protein Family: Proteasome activator
• UniProt Gene Name: PSME3
• UniProt Synonym Gene Names: REG-gamma; PA28g; PA28gamma
• UniProt Entry Name: PSME3_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]

Uniprot Description

PSME3: a protein implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome. Homohexamer. Significantly elevated in colorectal cancer patients compared with healthy donors and patients with benign bowel disease. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Proteasome complex; Protease

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: nucleoplasm; proteasome complex; proteasome activator complex; membrane; cytoplasm; nucleus; cytosol

Molecular Function: protein binding; p53 binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis