Typical Testing Data/Standard Curve (for reference only)
Human Prostaglandin Reductase 2 (PTGR2) ELISA Kit | PTGR2 elisa kit
Human Prostaglandin Reductase 2 (PTGR2) ELISA Kit
Gene Names
PTGR2; PGR2; ZADH1; HEL-S-298
Reactivity
Human
Synonyms
Prostaglandin Reductase 2 (PTGR2); Human Prostaglandin Reductase 2 (PTGR2) ELISA Kit; ZADH1; Zinc Binding Alcohol Dehydrogenase Domain Containing 1; 15-oxoprostaglandin 13-reductase; Zinc-binding alcohol dehydrogenase domain-containing protein 1; PTGR2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of PTCH1. No significant cross-reactivity or interference between PTCH1 and analogues was observed.
Sequence Length
351
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Rat Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
0.054ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PTCH1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PTCH1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PTGR2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PTCH1 in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PTCH1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PTCH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTCH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PTCH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to PTCH1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to PTCH1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PTCH1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PTCH1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for PTGR2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,675 Da
NCBI Official Full Name
prostaglandin reductase 2
NCBI Official Synonym Full Names
prostaglandin reductase 2
NCBI Official Symbol
PTGR2
NCBI Official Synonym Symbols
PGR2; ZADH1; HEL-S-298
NCBI Protein Information
prostaglandin reductase 2
UniProt Protein Name
Prostaglandin reductase 2
Protein Family
UniProt Gene Name
PTGR2
UniProt Synonym Gene Names
ZADH1; PRG-2
NCBI Description
This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Uniprot Description
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation ().
Research Articles on PTGR2
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Product Notes
The Human PTGR2 ptgr2 (Catalog #AAA2104925) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104925 ELISA Kit recognizes Human PTGR2. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the PTGR2 ptgr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prostaglandin Reductase 2 (PTGR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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