Human Programmed Cell Death Protein 4 (PDCD4) ELISA Kit | PDCD4 elisa kit

Human Programmed Cell Death Protein 4 (PDCD4) ELISA Kit

Cat. Num. AAA2706884

• Gene Names: PDCD4; H731
• Reactivity: Human
• Synonyms: Programmed Cell Death Protein 4 (PDCD4); Human Programmed Cell Death Protein 4 (PDCD4) ELISA Kit; H731; Neoplastic Transformation Inhibitor; Nuclear antigen H731-like; Protein 197/15a; PDCD4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Programmed Cell Death Protein 4 (PDCD4).; No significant cross-reactivity or interference between Programmed Cell Death Protein 4 (PDCD4) and analogues was observed.
• Sequence Length: 455

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Programmed Cell Death Protein 4 (PDCD4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Programmed Cell Death Protein 4 (PDCD4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDCD4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 4 (PDCD4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 4 (PDCD4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 4 (PDCD4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 4 (PDCD4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 313760537
• NCBI GeneID: 27250
• NCBI Accession #: NP_001186421.1
• NCBI GenBank Nucleotide #: NM_001199492.1
• Molecular Weight: 50,576 Da
• NCBI Official Full Name: programmed cell death protein 4 isoform 3
• NCBI Official Synonym Full Names: programmed cell death 4
• NCBI Official Symbol: PDCD4
• NCBI Official Synonym Symbols: H731
• NCBI Protein Information: programmed cell death protein 4
• UniProt Protein Name: Programmed cell death protein 4
• Protein Family: Programmed cell death protein
• UniProt Gene Name: PDCD4
• UniProt Synonym Gene Names: H731

NCBI Description

This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]

Uniprot Description

PDCD4: protein localized to the nucleus in proliferating cells that seems to possess a tumor suppressor activity. Directly interacts with the RNA helicase eIF4A and inhibits protein synthesis by interfering with the assembly of the cap-dependent translation initiation complex. Suppresses carbonic anhydrase type II protein expression in carcinoid cell lines. Since tumor cells require a high bicarbonate flux for their growth, carbonic anhydrase suppression results in growth inhibition. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two differentially spliced isoforms have been identified.

Protein type: Apoptosis; Tumor suppressor

Chromosomal Location of Human Ortholog: 10q25.2

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; RNA binding

Biological Process: apoptosis; BMP signaling pathway; cell aging; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of cytokine production involved in inflammatory response; negative regulation of JNK activity; negative regulation of myofibroblast differentiation; negative regulation of transcription, DNA-dependent; negative regulation of vascular smooth muscle cell differentiation; positive regulation of endothelial cell apoptotic process; positive regulation of inflammatory response; positive regulation of NIK/NF-kappaB signaling; positive regulation of smooth muscle cell apoptotic process