Related Product Information for HERC1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate HERC1 in samples. An antibody specific for HERC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHERC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HERC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HERC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI Description
This gen encodes a member of the HERC protein family. This protein stimulates guanine nucleotide exchange on ARF1 and Rab proteins. This protein may be involved in membrane transport processes. [provided by RefSeq, Mar 2012]
Uniprot Description
HERC1: Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin- protein ligase which accepts ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.
Protein type: EC 6.3.2.-; EC 6.3.2.19; GEF; GEF, ARF; Ligase; Ubiquitin conjugating system; Ubiquitin ligase
Chromosomal Location of Human Ortholog: 15q22.31
Cellular Component: cytoplasm; cytosol; Golgi apparatus; membrane
Molecular Function: ARF guanyl-nucleotide exchange factor activity; ubiquitin-protein transferase activity
Biological Process: cerebellar Purkinje cell differentiation; negative regulation of autophagy; neuromuscular process controlling balance; neuron projection development; positive regulation of GTPase activity; protein ubiquitination; transport
Disease: Macrocephaly, Dysmorphic Facies, And Psychomotor Retardation
Product Notes
The Human HERC1 herc1 (Catalog #
AAA280560) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280560 ELISA Kit recognizes Human HERC1.
It is sometimes possible for the material contained within the vial of
"Probable E3 ubiquitin-protein ligase HERC1, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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