Human Probable carboxypeptidase PM20D1 ELISA Kit | PM20D1 elisa kit
Human Probable carboxypeptidase PM20D1 (PM20D1) ELISA Kit
Gene Names
PM20D1; Cps1
Reactivity
Human
Synonyms
Probable carboxypeptidase PM20D1; Human Probable carboxypeptidase PM20D1 (PM20D1) ELISA Kit; PM20D1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PM20D1. No significant cross-reactivity or interference between Human PM20D1 and analogues was observed.
Sequence Length
502
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.156 ng/mL - 10 ng/mL
Sensitivity
0.052 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for PM20D1 elisa kit
Intended Uses: For the quantitative detection of Human Probable carboxypeptidase PM20D1 (PM20D1) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PM20D1 in Human serum, plasma. An antibody specific for PM20D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PM20D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PM20D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PM20D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PM20D1 in Human serum, plasma. An antibody specific for PM20D1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PM20D1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PM20D1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PM20D1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
39,544 Da
NCBI Official Full Name
N-fatty-acyl-amino acid synthase/hydrolase PM20D1
NCBI Official Synonym Full Names
peptidase M20 domain containing 1
NCBI Official Symbol
PM20D1
NCBI Official Synonym Symbols
Cps1
NCBI Protein Information
N-fatty-acyl-amino acid synthase/hydrolase PM20D1; probable carboxypeptidase PM20D1
UniProt Protein Name
N-fatty-acyl-amino acid synthase/hydrolase PM20D1
Protein Family
UniProt Gene Name
PM20D1
Uniprot Description
PM20D1: Belongs to the peptidase M20A family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.17.-; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q32.1
Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; lyase activity; metal ion binding; peptidase activity
Biological Process: amide biosynthetic process; amino acid metabolic process; cellular amide catabolic process; cellular lipid metabolic process; proteolysis
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Product Notes
The Human PM20D1 pm20d1 (Catalog #AAA280518) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280518 ELISA Kit recognizes Human PM20D1. It is sometimes possible for the material contained within the vial of "Probable carboxypeptidase PM20D1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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