Human Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit | PSG2 elisa kit

Human Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit

Cat. Num. AAA2000181

• Gene Names: PSG2; CEA; PSG1; PSBG2
• Reactivity: Human
• Synonyms: Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2); Human Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) ELISA Kit; CEA; PSG1; PSBG2; PSGGB; Carcinoembryonic Antigen SG8; Pregnancy-specific glycoprotein 2; Pregnancy-specific beta-1 glycoprotein E; PSG2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2).; No significant cross-reactivity or interference between Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) and analogues was observed.
• Sequence Length: 335

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Breast milk, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.78 -50ng/mL
• Sensitivity: < 0.34ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PSG2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pregnancy Specific Beta-1-Glycoprotein 2 (PSG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PSG2 elisa kit

Reproductive science

NCBI and Uniprot Product Information

• NCBI GI #: 156616282
• NCBI GeneID: 5670
• NCBI Accession #: NP_112536.2
• NCBI GenBank Nucleotide #: NM_031246.3
• UniProt Accession #: P11465; Q8TCD9; Q9UEA4; Q9UQ78
• Molecular Weight: 37,216 Da
• NCBI Official Full Name: pregnancy-specific beta-1-glycoprotein 2
• NCBI Official Synonym Full Names: pregnancy specific beta-1-glycoprotein 2
• NCBI Official Symbol: PSG2
• NCBI Official Synonym Symbols: CEA; PSG1; PSBG2
• NCBI Protein Information: pregnancy-specific beta-1-glycoprotein 2
• UniProt Protein Name: Pregnancy-specific beta-1-glycoprotein 2
• Protein Family: Pregnancy-specific beta-1-glycoprotein
• UniProt Gene Name: PSG2
• UniProt Synonym Gene Names: PSBG2; PS-beta-G-2; PSBG-2; Pregnancy-specific glycoprotein 2; PS-beta-E
• UniProt Entry Name: PSG2_HUMAN

NCBI Description

The human pregnancy-specific glycoproteins (PSGs) are a family of proteins that are synthesized in large amounts by placental trophoblasts and released into the maternal circulation during pregnancy. Molecular cloning and analysis of several PSG genes has indicated that the PSGs form a subgroup of the carcinoembryonic antigen (CEA) gene family, which belongs to the immunoglobulin superfamily of genes. Members of the CEA family consist of a single N domain, with structural similarity to the immunoglobulin variable domains, followed by a variable number of immunoglobulin constant-like A and/or B domains. Most PSGs have an arg-gly-asp (RGD) motif, which has been shown to function as an adhesion recognition signal for several integrins, in the N-terminal domain (summary by Teglund et al., 1994 [PubMed 7851896]). For additional general information about the PSG gene family, see PSG1 (MIM 176390).[supplied by OMIM, Oct 2009]

Uniprot Description

PSG2: Belongs to the immunoglobulin superfamily. CEA family

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: extracellular region

Biological Process: cell migration; female pregnancy