Human Prefoldin Subunit 1 (PFDN1) ELISA Kit | PFDN1 elisa kit

Human Prefoldin Subunit 1 (PFDN1) ELISA Kit

Cat. Num. AAA2024279

• Gene Names: PFDN1; PDF; PFD1
• Reactivity: Human
• Synonyms: Prefoldin Subunit 1 (PFDN1); Human Prefoldin Subunit 1 (PFDN1) ELISA Kit; PDF; PFD1; PFDN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Prefoldin Subunit 1 (PFDN1).; No significant cross-reactivity or interference between Prefoldin Subunit 1 (PFDN1) and analogues was observed.
• Sequence Length: 122

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.112ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Prefoldin Subunit 1 (PFDN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Prefoldin Subunit 1 (PFDN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PFDN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prefoldin Subunit 1 (PFDN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Prefoldin Subunit 1 (PFDN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Prefoldin Subunit 1 (PFDN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Prefoldin Subunit 1 (PFDN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PFDN1 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 21536449
• NCBI GeneID: 5201
• NCBI Accession #: NP_002613.2
• NCBI GenBank Nucleotide #: NM_002622.4
• UniProt Accession #: O60925; Q53F95; Q96EX6; B2RD02
• Molecular Weight: 14,210 Da
• NCBI Official Full Name: prefoldin subunit 1
• NCBI Official Synonym Full Names: prefoldin subunit 1
• NCBI Official Symbol: PFDN1
• NCBI Official Synonym Symbols: PDF; PFD1
• NCBI Protein Information: prefoldin subunit 1
• UniProt Protein Name: Prefoldin subunit 1
• Protein Family: Prefoldin
• UniProt Gene Name: PFDN1
• UniProt Synonym Gene Names: PFD1
• UniProt Entry Name: PFD1_HUMAN

NCBI Description

This gene encodes a member of the prefoldin beta subunit family. The encoded protein is one of six subunits of prefoldin, a molecular chaperone complex that binds and stabilizes newly synthesized polypeptides, thereby allowing them to fold correctly. The complex, consisting of two alpha and four beta subunits, forms a double beta barrel assembly with six protruding coiled-coils. [provided by RefSeq, Jul 2008]

Uniprot Description

PFDN1: Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins. Belongs to the prefoldin subunit beta family.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: prefoldin complex

Molecular Function: unfolded protein binding; transcription factor activity

Biological Process: 'de novo' posttranslational protein folding; telencephalon development; cellular protein metabolic process; regulation of transcription, DNA-dependent; protein folding; B cell activation; cerebellum development; actin cytoskeleton organization and biogenesis; cell cycle