Typical Testing Data/Standard Curve (for reference only)
Human PRB1 ELISA Kit | PRB1 elisa kit
Human PRB1 (Basic Salivary Proline Rich Protein 1) ELISA Kit
Gene Names
PRB1; PM; PMF; PMS; PRB1L; PRB1M
Reactivity
Human
Synonyms
PRB1; Human PRB1 (Basic Salivary Proline Rich Protein 1) ELISA Kit; PRB1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human PRB1. No significant cross-reactivity or interference between Human PRB1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human PRB1 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
198
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.156-10ng/mL
Sensitivity
Min: 0.094ng/mL; Max: 10ng/mL
Preparation and Storage
Store at 4 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PRB1 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRB1 in human serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRB1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PRB1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PRB1. You can calculate the concentration of Human PRB1 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRB1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human PRB1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRB1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PRB1. You can calculate the concentration of Human PRB1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
38,546 Da
NCBI Official Full Name
PRB1 protein
NCBI Official Synonym Full Names
proline-rich protein BstNI subfamily 1
NCBI Official Symbol
PRB1
NCBI Official Synonym Symbols
PM; PMF; PMS; PRB1L; PRB1M
NCBI Protein Information
basic salivary proline-rich protein 1; BstNI type basic salivary proline-rich protein 1; PRB1 large length copy; basic proline-rich protein; parotid middle band protein; salivary protein Pe
UniProt Protein Name
Basic salivary proline-rich protein 1
Protein Family
UniProt Gene Name
PRB1
UniProt Synonym Gene Names
Salivary proline-rich protein
UniProt Entry Name
PRP1_HUMAN
NCBI Description
This gene encodes a precursor for proline-rich salivary proteins. This and five other genes that encode salivary proline-rich proteins (PRPs)form a PRP gene cluster in the chromosomal 12p13 region. Precursor salivary PRPs are cleaved multiple times to produce a diversity of secreted peptides. Alleles of this gene exhibit tandem repeat length variation in the coding region as well as polymorphic cleavage sites and polymorphic stop codons. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
PRB1:
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12p13.2
Cellular Component: extracellular region
Similar Products
Product Notes
The Human PRB1 prb1 (Catalog #AAA2500362) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2500362 ELISA Kit recognizes Human PRB1. It is sometimes possible for the material contained within the vial of "PRB1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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