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Typical Testing Data/Standard Curve (for reference only)

Human POSTN/OSF-2 (Periostin) ELISA Kit | POSTN/OSF-2 elisa kit

QuicKey Pro Human POSTN/OSF-2 (Periostin) ELISA Kit

Gene Names
POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin
Reactivity
Human
Synonyms
POSTN/OSF-2 (Periostin); QuicKey Pro Human POSTN/OSF-2 (Periostin) ELISA Kit; POSTN/OSF-2; POSTN/OSF-2 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This kit recognizes POSTN/OSF-2 in samples. No significant cross-reactivity or interference between POSTN/OSF-2 and analogues was observed.
This ELISA kit applies to the in vitro quantitative determination of POSTN/OSF-2 concentrations in serum and plasma.
Assay Type
Sandwich ELISA
Detection Method
Colormetric
Tested Sample Types
Serum and plasma
Detection Range
3.12-200ng/mL
Sensitivity
1.52ng/mL
Precision
Both intra-CV and inter-CV are < 10%.
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human POSTN/OSF-2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human POSTN/OSF-2 are added to the micro ELISA plate wells. Human POSTN/OSF-2 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to POSTN/OSF-2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450+/-2nm. The concentration of Human POSTN/OSF-2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Preparation and Storage
An unopened kit can be stored at 2-8? for 6 months. After test, the unused wells and reagents should be stored according to the table/manual.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
93,314 Da
NCBI Official Full Name
periostin isoform 1
NCBI Official Synonym Full Names
periostin, osteoblast specific factor
NCBI Official Symbol
POSTN
NCBI Official Synonym Symbols
PN; OSF2; OSF-2; PDLPOSTN; periostin
NCBI Protein Information
periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like)
UniProt Protein Name
Periostin
UniProt Gene Name
POSTN
UniProt Synonym Gene Names
OSF2; PN; OSF-2
UniProt Entry Name
POSTN_HUMAN

NCBI Description

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

POSTN: Binds to heparin. Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Cell adhesion; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q13.3

Cellular Component: extracellular matrix; proteinaceous extracellular matrix; extracellular space; trans-Golgi network

Molecular Function: heparin binding; protein binding; cell adhesion molecule binding

Biological Process: tissue development; extracellular matrix organization and biogenesis; regulation of Notch signaling pathway; cell adhesion; skeletal development

Research Articles on POSTN/OSF-2

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Product Notes

The Human POSTN/OSF-2 postn (Catalog #AAA2568095) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2568095 ELISA Kit recognizes Human POSTN/OSF-2. It is sometimes possible for the material contained within the vial of "POSTN/OSF-2 (Periostin), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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