Human PMN-Elastase ELISA Kit
PMN-Elastase ELISA
Reactivity
Human
Synonyms
PMN-Elastase; PMN-Elastase ELISA; PMN-Elastase elisa kit
Reactivity
Human
Specificity
No cross reactivity to other proteins in stool and serum was observed. Cross reactivity with PMN elastase as well as a good correlation with PMN elastase content in mouse serum was observed.
Samples
Stool, Urine, Serum And Plasma
Assay Type
Sandwich
Sensitivity
0,164 ng/ml.
Preparation and Storage
2 - 8 degree C.
Related Product Information for PMN-Elastase elisa kit
Intended Uses: The described ELISA is intended for the determination of EDN (eosinophil-derived neurotoxin, eosinophil protein x, EPX) in serum, plasma, urine and stool. For research use only.
Principle of the Assay: The assay utilizes the two-site sandwich ELISA technique with two selected antibodies (monoclonal and polyclonal) that bind to human EDN. Calibrator, controls and diluted samples which are assayed for EDN are added to wells of microplate coated with a high affine monoclonal anti-human EDN antibody. During the first incubation step, EDN in the samples is bound by the immobilized antibody. Then a peroxidase-labeled conjugate is added to each well and the following complex is formed: capture antibody-human EDN-peroxidase conjugate. Tetramethylbenzidine is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the EDN concentration of the sample. Samples are determined by referring their optical density to a lot-dependent master calibration curve and the use of the calibrator that is run with each test!!Background/Introduction: EDN (eosinophil-derived neurotoxin, eosinophil protein x, EPX), a cationic glycoprotein, which is released by activated eosinophils, has strong cytotoxic characteristics and plays a significant role in the prevention of virus infections. It is released by the eosinophil granules in places where eosinophils are mainly found: in the skin, lungs, urogenital and gastrointestinal tract, that is, in the organs acting as an entry point for pathogens. The accumulation of EDN in the intestine is associated with inflammation and tissue damage. Measuring of EDN in stool can serve as an objective parameter for a current chronic inflammation located in the gastrointestinal area. In the case of Colitis ulcerosa and Crohn's disease, the EDN measurement enables the evaluation of a disease's activity and the prediction of a relapse.
Principle of the Assay: The assay utilizes the two-site sandwich ELISA technique with two selected antibodies (monoclonal and polyclonal) that bind to human EDN. Calibrator, controls and diluted samples which are assayed for EDN are added to wells of microplate coated with a high affine monoclonal anti-human EDN antibody. During the first incubation step, EDN in the samples is bound by the immobilized antibody. Then a peroxidase-labeled conjugate is added to each well and the following complex is formed: capture antibody-human EDN-peroxidase conjugate. Tetramethylbenzidine is used as a substrate for peroxidase. Finally, an acidic stop solution is added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the EDN concentration of the sample. Samples are determined by referring their optical density to a lot-dependent master calibration curve and the use of the calibrator that is run with each test!!Background/Introduction: EDN (eosinophil-derived neurotoxin, eosinophil protein x, EPX), a cationic glycoprotein, which is released by activated eosinophils, has strong cytotoxic characteristics and plays a significant role in the prevention of virus infections. It is released by the eosinophil granules in places where eosinophils are mainly found: in the skin, lungs, urogenital and gastrointestinal tract, that is, in the organs acting as an entry point for pathogens. The accumulation of EDN in the intestine is associated with inflammation and tissue damage. Measuring of EDN in stool can serve as an objective parameter for a current chronic inflammation located in the gastrointestinal area. In the case of Colitis ulcerosa and Crohn's disease, the EDN measurement enables the evaluation of a disease's activity and the prediction of a relapse.
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Product Notes
The Human PMN-Elastase (Catalog #AAA495098) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495098 ELISA Kit recognizes Human PMN-Elastase. It is sometimes possible for the material contained within the vial of "PMN-Elastase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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