Human PMN-Elastase ELISA Kit
PMN-Elastase ELISA
Reactivity
Human
Synonyms
PMN-Elastase; PMN-Elastase ELISA; PMN-Elastase elisa kit
Reactivity
Human
Specificity
No cross reactivity to other proteins in stool and serum was observed. Cross reactivity with PMN elastase as well as a good correlation with PMN elastase content in mouse serum was observed.
Samples
Stool
Preparation and Storage
2 - 8 degree C.
Related Product Information for PMN-Elastase elisa kit
Intended Uses: The described enzyme-linked immunosorbent assay (ELISA) is intended for the determination of PMN elastase in stool. It is for research use only.
Principle of the Assay: In a first incubation step, PMN elastase in the sample is bound to polyclonal sheepanti-PMN elastase antibodies, which are immobilized on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody is added. This antibody is able to detect both the free and the complexed form with the specific inhibitor (alpha1-proteinase inhibitor = alpha1-antitrypsin). The determination of the bound PMN elastase is carried out by adding an anti-mouse peroxidase-labeled conjugate. Finally, the PMN elastase-antigen-antibody-complex is incubated with the peroxidase substrate, tetramethylbenzidine. An acidic stop solution is then added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of PMN elastase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standards. PMN elastase, present in the samples, is determined directly from this curve!!Background/Introduction: PMN elastase from human polymorphnuclear granulocytes is a glycoprotein of 30 kDa which belongs to the group of serine proteases. Active PMN elastase is released from azurophil granula of neutrophil granulocytes after irritation or disintegration. The determination of the PMN elastase in stool is used to record inflammatory reactions in which neutrophils are involved. Especially in Crohn´s disease, the inflammatory process is accompanied by an increased phagocytic activity and the biological decay of the phagocytic cells, which leads to an increased release of PMN elastase and other lysosomal enzymes.
Principle of the Assay: In a first incubation step, PMN elastase in the sample is bound to polyclonal sheepanti-PMN elastase antibodies, which are immobilized on the surface of the microtiter wells. To remove all unbound substances, a washing step is carried out. In a second incubation step, a monoclonal mouse-anti-PMN elastase antibody is added. This antibody is able to detect both the free and the complexed form with the specific inhibitor (alpha1-proteinase inhibitor = alpha1-antitrypsin). The determination of the bound PMN elastase is carried out by adding an anti-mouse peroxidase-labeled conjugate. Finally, the PMN elastase-antigen-antibody-complex is incubated with the peroxidase substrate, tetramethylbenzidine. An acidic stop solution is then added to terminate the reaction. The color changes from blue to yellow. The intensity of the yellow color is directly proportional to the concentration of PMN elastase in the sample. A dose response curve of the absorbance unit (optical density, OD) vs. concentration is generated, using the values obtained from the standards. PMN elastase, present in the samples, is determined directly from this curve!!Background/Introduction: PMN elastase from human polymorphnuclear granulocytes is a glycoprotein of 30 kDa which belongs to the group of serine proteases. Active PMN elastase is released from azurophil granula of neutrophil granulocytes after irritation or disintegration. The determination of the PMN elastase in stool is used to record inflammatory reactions in which neutrophils are involved. Especially in Crohn´s disease, the inflammatory process is accompanied by an increased phagocytic activity and the biological decay of the phagocytic cells, which leads to an increased release of PMN elastase and other lysosomal enzymes.
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Product Notes
The Human PMN-Elastase (Catalog #AAA495097) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA495097 ELISA Kit recognizes Human PMN-Elastase. It is sometimes possible for the material contained within the vial of "PMN-Elastase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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