Human Platelet Activating Factor Receptor (PAFR) ELISA Kit | PAFR elisa kit

Human Platelet Activating Factor Receptor (PAFR) ELISA Kit

Cat. Num. AAA2704657

• Gene Names: PTAFR; PAFR
• Reactivity: Human
• Synonyms: Platelet Activating Factor Receptor (PAFR); Human Platelet Activating Factor Receptor (PAFR) ELISA Kit; PTAFR; PAFR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Platelet Activating Factor Receptor (PAFR).; No significant cross-reactivity or interference between Platelet Activating Factor Receptor (PAFR) and analogues was observed.
• Sequence Length: 342

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Platelet Activating Factor Receptor (PAFR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Platelet Activating Factor Receptor (PAFR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PAFR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Platelet Activating Factor Receptor (PAFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Platelet Activating Factor Receptor (PAFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Platelet Activating Factor Receptor (PAFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Platelet Activating Factor Receptor (PAFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4506241
• NCBI GeneID: 5724
• NCBI Accession #: NP_000943.1
• NCBI GenBank Nucleotide #: NM_000952.4
• UniProt Accession #: P25105; A3KMC8; A8K2H5
• Molecular Weight: 39,203 Da
• NCBI Official Full Name: platelet-activating factor receptor
• NCBI Official Synonym Full Names: platelet activating factor receptor
• NCBI Official Symbol: PTAFR
• NCBI Official Synonym Symbols: PAFR
• NCBI Protein Information: platelet-activating factor receptor
• UniProt Protein Name: Platelet-activating factor receptor
• UniProt Gene Name: PTAFR
• UniProt Synonym Gene Names: PAFR; PAF-R; PAFr

NCBI Description

This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

PTAFR: Receptor for platelet activating factor, a chemotactic phospholipid mediator that possesses potent inflammatory, smooth- muscle contractile and hypotensive activity. Seems to mediate its action via a G protein that activates a phosphatidylinositol- calcium second messenger system. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: integral component of membrane; integral component of plasma membrane; membrane; plasma membrane; secretory granule membrane

Molecular Function: G-protein coupled purinergic nucleotide receptor activity; G-protein coupled receptor activity; lipopolysaccharide binding; lipopolysaccharide receptor activity; mitogen-activated protein kinase binding; phospholipid binding; platelet activating factor receptor activity

Biological Process: chemotaxis; cytokine production; G-protein coupled receptor signaling pathway; immune response; inflammatory response; inositol trisphosphate biosynthetic process; lipopolysaccharide-mediated signaling pathway; negative regulation of blood pressure; neutrophil degranulation; parturition; phosphoinositide-mediated signaling; positive regulation of interleukin-6 biosynthetic process; positive regulation of neutrophil degranulation; positive regulation of smooth muscle cell proliferation; positive regulation of translation; positive regulation of tumor necrosis factor production; positive regulation of vasoconstriction; regulation of transcription from RNA polymerase II promoter; response to symbiotic bacterium; transcytosis