Typical Testing Data/Standard Curve (for reference only)
Human Pigment epithelium-derived factor precursor, Pre-PEDF ELISA Kit | Pre-PEDF elisa kit
Human Pigment epithelium-derived factor precursor, Pre-PEDF ELISA Kit
Gene Names
SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35
Reactivity
Human
Synonyms
Pigment epithelium-derived factor precursor; Pre-PEDF; Human Pigment epithelium-derived factor precursor; Pre-PEDF ELISA Kit; Pre-PEDF elisa kit
Reactivity
Human
Sequence Length
418
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.05ng/ml-15ng/ml
Sensitivity
0.014ng/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Pre-PEDF elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Phosphorylated Type of PI3K (also known as P-PI3K) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human P-PI3K antibody. P-PI3K present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human P-PI3K Antibody is added and binds to P-PI3K in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated P-PI3K antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human P-PI3K. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human P-PI3K antibody. P-PI3K present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human P-PI3K Antibody is added and binds to P-PI3K in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated P-PI3K antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human P-PI3K. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
46,312 Da
NCBI Official Full Name
pigment epithelium-derived factor isoform 1
NCBI Official Synonym Full Names
serpin family F member 1
NCBI Official Symbol
SERPINF1
NCBI Official Synonym Symbols
OI6; OI12; PEDF; EPC-1; PIG35
NCBI Protein Information
pigment epithelium-derived factor
UniProt Protein Name
Pigment epithelium-derived factor
UniProt Gene Name
SERPINF1
UniProt Synonym Gene Names
PEDF; PEDF
NCBI Description
This gene encodes a member of the serpin family that does not display the serine protease inhibitory activity shown by many of the other serpin proteins. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI. [provided by RefSeq, Aug 2016]
Uniprot Description
Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
Research Articles on Pre-PEDF
Similar Products
Product Notes
The Human Pre-PEDF serpinf1 (Catalog #AAA161086) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161086 ELISA Kit recognizes Human Pre-PEDF. It is sometimes possible for the material contained within the vial of "Pigment epithelium-derived factor precursor, Pre-PEDF, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
