Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoglucomutase-2 (PGM2) Standard Curve.)
Human Phosphoglucomutase-2 (PGM2) ELISA Kit | PGM2 elisa kit
Human Phosphoglucomutase-2 (PGM2) ELISA Kit
Gene Names
PGM2; MSTP006
Reactivity
Human
Synonyms
Phosphoglucomutase-2 (PGM2); Human Phosphoglucomutase-2 (PGM2) ELISA Kit; PGM2; FLJ10983; MSTP006; glucose phosphomutase 2; PGM2 elisa kit
Reactivity
Human
Specificity
Human Phosphoglucomutase-2 (PGM2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PGM2. No significant cross-reactivity or interference between Human PGM2 and analogues was observed.
Sequence Length
612
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoglucomutase-2 (PGM2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Phosphoglucomutase-2 (PGM2) Standard Curve.)
Related Product Information for PGM2 elisa kit
Description: This Human Phosphoglucomutase-2 (PGM2) ELISA Kit employs a two-site sandwich ELISA to quantitate PGM2 in samples. An antibody specific for PGM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The PGM2 protein shares about 20% identity with mammalian PGM1. Sequence analysis suggested that PGM2 is a cytosolic protein. Quantitative RT-PCR of mouse tissues detected the highest expression levels of PGM2 in lung, spleen, and thymus. PGM2 acted more than 10-fold better as a phosphopentomutasethan as a phosphoglucomutase. PGM2 may play a role in congenital immunodeficiencies. phosphopentomutase uses ribose 1-phosphate and deoxyribose 1-phosphate, which are formed by purine nucleoside phosphorylase and uridine phosphorylase, and that the absence of phosphopentomutase should result in the accumulation of ribose 1-phosphate and deoxyribose 1-phosphate and therefore in a functional block of purine nucleoside phosphorylase.
Background: The PGM2 protein shares about 20% identity with mammalian PGM1. Sequence analysis suggested that PGM2 is a cytosolic protein. Quantitative RT-PCR of mouse tissues detected the highest expression levels of PGM2 in lung, spleen, and thymus. PGM2 acted more than 10-fold better as a phosphopentomutasethan as a phosphoglucomutase. PGM2 may play a role in congenital immunodeficiencies. phosphopentomutase uses ribose 1-phosphate and deoxyribose 1-phosphate, which are formed by purine nucleoside phosphorylase and uridine phosphorylase, and that the absence of phosphopentomutase should result in the accumulation of ribose 1-phosphate and deoxyribose 1-phosphate and therefore in a functional block of purine nucleoside phosphorylase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phosphoglucomutase-2
NCBI Official Synonym Full Names
phosphoglucomutase 2
NCBI Official Symbol
PGM2
NCBI Official Synonym Symbols
MSTP006
NCBI Protein Information
phosphoglucomutase-2
UniProt Protein Name
Phosphoglucomutase-2
Protein Family
UniProt Gene Name
PGM2
UniProt Synonym Gene Names
PGM 2
UniProt Entry Name
PGM2_HUMAN
Uniprot Description
PGM2: Catalyzes the conversion of the nucleoside breakdown products ribose-1-phosphate and deoxyribose-1-phosphate to the corresponding 5-phosphopentoses. May also catalyze the interconversion of glucose-1-phosphate and glucose-6-phosphate. Has low glucose 1,6-bisphosphate synthase activity. Belongs to the phosphohexose mutase family.
Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - starch and sucrose; EC 5.4.2.2; EC 5.4.2.7; Isomerase
Chromosomal Location of Human Ortholog: 4p14
Cellular Component: cytosol
Molecular Function: phosphoglucomutase activity; phosphopentomutase activity; protein binding
Biological Process: galactose catabolic process; glycogen biosynthetic process; glycogen catabolic process; pentose-phosphate shunt
Research Articles on PGM2
Similar Products
Product Notes
The Human PGM2 pgm2 (Catalog #AAA9714891) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714891 ELISA Kit recognizes Human PGM2. It is sometimes possible for the material contained within the vial of "Phosphoglucomutase-2 (PGM2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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