Human phospho Protein Kinase C alpha (P-PKCa) ELISA Kit | P-PKCa elisa kit

Human phospho Protein Kinase C alpha (P-PKCa) ELISA Kit

Cat. Num. AAA744355

• Gene Names: PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
• Reactivity: Human
• Synonyms: phospho Protein Kinase C alpha (P-PKCa); Human phospho Protein Kinase C alpha (P-PKCa) ELISA Kit; P-PKCa elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 U/Ml
• Intended Uses: This pAMPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human pAMPK. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for P-PKCa elisa kit

Principle of the Assay: pAMPK ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-pAMPK antibody and an pAMPK-HRP conjugate. The assay sample and buffer are incubated together with pAMPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pAMPK concentration since pAMPK from samples and pAMPK-HRP conjugate compete for the anti-pAMPK antibody binding site. Since the number of sites is limited, as more sites are occupied by pAMPK from the sample, fewer sites are left to bind pAMPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pAMPK concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31544941
• NCBI GeneID: 5578
• UniProt Accession #: P17252; Q15137; Q32M72; Q96RE4; B5BU22
• Molecular Weight: 76,750 Da
• NCBI Official Full Name: PRKCA protein, partial
• NCBI Official Synonym Full Names: protein kinase C, alpha
• NCBI Official Symbol: PRKCA
• NCBI Official Synonym Symbols: AAG6; PKCA; PRKACA; PKC-alpha
• NCBI Protein Information: protein kinase C alpha type; PKC-A; aging-associated gene 6
• UniProt Protein Name: Protein kinase C alpha type
• UniProt Gene Name: PRKCA
• UniProt Synonym Gene Names: PKCA; PRKACA; PKC-A; PKC-alpha
• UniProt Entry Name: KPCA_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.

Protein type: Kinase, protein; Protein kinase, AGC; EC 2.7.11.13; Protein kinase, Ser/Thr (non-receptor); Oncoprotein; AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 17q22-q23.2

Cellular Component: nucleoplasm; photoreceptor outer segment; cell soma; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; apical part of cell; dendrite; cytoplasm; mitochondrial membrane; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; enzyme binding; zinc ion binding; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; regulation of muscle contraction; positive regulation of lipopolysaccharide-mediated signaling pathway; mitotic nuclear envelope disassembly; negative regulation of insulin receptor signaling pathway; positive regulation of mitotic cell cycle; signal transduction; protein amino acid phosphorylation; induction of positive chemotaxis; synaptic transmission; negative regulation of cell proliferation; chondrocyte differentiation; angiogenesis; cell adhesion; positive regulation of macrophage differentiation; regulation of peptidyl-tyrosine phosphorylation; regulation of the force of heart contraction; inactivation of MAPK activity; epidermal growth factor receptor signaling pathway; neutrophil chemotaxis; platelet activation; fibroblast growth factor receptor signaling pathway; positive regulation of blood vessel endothelial cell migration; negative regulation of adenylate cyclase activity; adenylate cyclase activation; cellular calcium ion homeostasis; rhodopsin mediated signaling; positive regulation of angiogenesis; negative regulation of glucose import; regulation of rhodopsin mediated signaling; phospholipase C activation; energy reserve metabolic process; innate immune response; gene expression; positive regulation of endothelial cell proliferation; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; regulation of insulin secretion; positive regulation of cell migration; positive regulation of inflammatory response