Human Pescadillo Homolog 1 (PES1) ELISA Kit | PES1 elisa kit

Human Pescadillo Homolog 1 (PES1) ELISA Kit

Cat. Num. AAA2025054

• Gene Names: PES1; PES
• Reactivity: Human
• Synonyms: Pescadillo Homolog 1 (PES1); Human Pescadillo Homolog 1 (PES1) ELISA Kit; PES1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pescadillo Homolog 1 (PES1).; No significant cross-reactivity or interference between Pescadillo Homolog 1 (PES1) and analogues was observed.
• Sequence Length: 588

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.063ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pescadillo Homolog 1 (PES1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pescadillo Homolog 1 (PES1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PES1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pescadillo Homolog 1 (PES1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pescadillo Homolog 1 (PES1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pescadillo Homolog 1 (PES1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pescadillo Homolog 1 (PES1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PES1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 7657455
• NCBI GeneID: 23481
• NCBI Accession #: NP_055118.1
• NCBI GenBank Nucleotide #: NM_014303.3
• Molecular Weight: 67,456 Da
• NCBI Official Full Name: pescadillo homolog isoform 1
• NCBI Official Synonym Full Names: pescadillo ribosomal biogenesis factor 1
• NCBI Official Symbol: PES1
• NCBI Official Synonym Symbols: PES
• NCBI Protein Information: pescadillo homolog
• UniProt Protein Name: Pescadillo homolog
• UniProt Gene Name: PES1
• UniProt Entry Name: PESC_HUMAN

NCBI Description

This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9. [provided by RefSeq, Aug 2011]

Uniprot Description

PES1: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Belongs to the pescadillo family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; RNA-binding; Translation

Chromosomal Location of Human Ortholog: 22q12.1

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; condensed chromosome; nucleus

Molecular Function: ribonucleoprotein binding; protein binding

Biological Process: cell proliferation; regulation of cell cycle; protein localization in organelle; maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); ribosomal large subunit biogenesis and assembly; rRNA processing; nucleolus organization and biogenesis