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Human Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) ELISA Kit | ACOX1 elisa kit

Human Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) ELISA Kit

Gene Names
ACOX1; ACOX; SCOX; PALMCOX
Reactivity
Human
Synonyms
Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1); Human Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1) ELISA Kit; ACOX; MGC1198; PALMCOX; SCOX; acyl-CoA oxidase; straight-chain; acyl-Coenzyme A oxidase 1; peroxisomal fatty acyl-CoA oxidase; ACOX1 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human ACOX1. No significant cross-reactivity or interference between Human ACOX1 and analogues was observed.
Asssay Type
Quantitive Sandwich
Sample Types
Serum, Plasma, Other biological fluids
Detection Wavelength
450nm
Sample Volume
1-200uL
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
Test Principle
This assay employs a two-site sandwich ELISA to quantitate ACOX1 in samples. An antibody specific for ACOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACOX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Assay Duration
1-4.5h
Preparation and Storage
Store at 2-8 degree C

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
51
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,668 Da
NCBI Official Full Name
peroxisomal acyl-coenzyme A oxidase 1 isoform a
NCBI Official Synonym Full Names
acyl-CoA oxidase 1
NCBI Official Symbol
ACOX1
NCBI Official Synonym Symbols
ACOX; SCOX; PALMCOX
NCBI Protein Information
peroxisomal acyl-coenzyme A oxidase 1
UniProt Protein Name
Peroxisomal acyl-coenzyme A oxidase 1
UniProt Gene Name
ACOX1
UniProt Synonym Gene Names
AOX1 PublicationManual assertion based on opinion iniRef.4; SCOX

NCBI Description

The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. Isoform 1 shows highest activity against medium-chain fatty acyl-CoAs and activity decreases with increasing chain length. Isoform 2 is active against a much broader range of substrates and shows activity towards very long-chain acyl-CoAs. Isoform 2 is twice as active as isoform 1 against 16-hydroxy-palmitoyl-CoA and is 25% more active against 1,16-hexadecanodioyl-CoA.

Research Articles on ACOX1

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Product Notes

The Human ACOX1 acox1 (Catalog #AAA2801667) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2801667 ELISA Kit recognizes Human ACOX1. It is sometimes possible for the material contained within the vial of "Peroxisomal acyl-coenzyme A oxidase 1 (ACOX1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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